ArticleEMBO molecular medicine2014
Cyclic-di-GMP signalling and biofilm-related properties of the Shiga toxin-producing 2011 German outbreak Escherichia coli O104:H4.
Article in EMBO molecular medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 63 citations in OpenAlex.
- EnteroaggregativeEcoSal Plus · 2025Review
- A challenging STEC strain isolation from patients' stools: an O166:H15 STEC strain with theMicrobiology spectrum · 2024Article
- Fluoropyrimidines affectBiofilm · 2024Article
- A convergent evolutionary pathway attenuating cellulose production drives enhanced virulence of some bacteria.Nature communications · 2024Article
- Characterisation of Variants of Cyclic di-GMP Turnover Proteins Associated with Semi-Constitutive rdar Morphotype Expression in Commensal and UropathogenicMicroorganisms · 2023Article
- Shiga-Toxin-Producing Strains ofMicroorganisms · 2023Article
- Evolution of cyclic di-GMP signalling on a short and long term time scale.Microbiology (Reading, England) · 2023Article
- Shigella flexneri Diguanylate Cyclases Regulate Virulence.Journal of bacteriology · 2021Article
- The Deletion ofJournal of bacteriology · 2021Article
- Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies.Microbial cell (Graz, Austria) · 2021Review
- A Single Nucleotide Polymorphism inFrontiers in microbiology · 2021Article
- Article
- PchE Regulation ofInternational journal of molecular sciences · 2020Article
- Metabolic Traits of Bovine Shiga Toxin-ProducingToxins · 2020Article
- Evaluation of Phosphoethanolamine Cellulose Production among Bacterial Communities Using Congo Red Fluorescence.Journal of bacteriology · 2020Article
- Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG.Molecules (Basel, Switzerland) · 2019Review
- Nanoemulsion-loaded hydrogel coatings for inhibition of bacterial virulence and biofilm formation on solid surfaces.Scientific reports · 2019Article
- Macrocolony of NDM-1 ProducingPathogens (Basel, Switzerland) · 2019Article
- Article
- Phosphoethanolamine cellulose enhances curli-mediated adhesion of uropathogenicProceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In 2011, nearly 4,000 people in Germany were infected by Shiga toxin (Stx)-producing Escherichia coli O104:H4 with > 22% of patients developing haemolytic uraemic syndrome (HUS). Genome sequencing showed the outbreak strain to be related to enteroaggregative E. coli (EAEC), suggesting its high virulence results from EAEC-typical strong adherence and biofilm formation combined to Stx production. Here, we report that the outbreak strain contains a novel diguanylate cyclase (DgcX)--producing the biofilm-promoting second messenger c-di-GMP--that shows higher expression than any other known E. coli diguanylate cyclase. Unlike closely related E. coli, the outbreak strain expresses the c-di-GMP-controlled biofilm regulator CsgD and amyloid curli fibres at 37°C, but is cellulose-negative. Moreover, it constantly generates derivatives with further increased and deregulated production of CsgD and curli. Since curli fibres are strongly proinflammatory, with cellulose counteracting this effect, high c-di-GMP and curli production by the outbreak O104:H4 strain may enhance not only adherence but may also contribute to inflammation, thereby facilitating entry of Stx into the bloodstream and to the kidneys where Stx causes HUS.
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