Evidence map›Paper›PMID 25360525›Full record

ArticlePloS one2014

Development of a standardized and safe airborne antibacterial assay, and its evaluation on antibacterial biomimetic model surfaces.

Ali Al-Ahmad, Peng Zou, Diana Lorena Guevara Solarte, Elmar Hellwig, Thorsten Steinberg, Karen Lienkamp

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 29 citations in OpenAlex.

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  5. Degradable Polymer Films Made from Poly(salicylic-acid-Macromolecular chemistry and physics · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ali Al-AhmadDepartment of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert-Ludwigs-Universität, Freiburg, Germany.
Peng ZouFreiburg Institute for Advanced Studies (FRIAS), Albert-Ludwigs-Universität, Freiburg, Germany; Department of Microsystems Engineering (IMTEK), Albert-Ludwigs-Universität, Freiburg, Germany.
Diana Lorena Guevara SolarteDepartment of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert-Ludwigs-Universität, Freiburg, Germany; Department of Microsystems Engineering (IMTEK), Albert-Ludwigs-Universität, Freiburg, Germany.
Elmar HellwigDepartment of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert-Ludwigs-Universität, Freiburg, Germany.
Thorsten SteinbergOral Biotechnology, University Medical Center of the Albert-Ludwigs-Universität, Freiburg, Germany.
Karen LienkampFreiburg Institute for Advanced Studies (FRIAS), Albert-Ludwigs-Universität, Freiburg, Germany; Department of Microsystems Engineering (IMTEK), Albert-Ludwigs-Universität, Freiburg, Germany.
University of Freiburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial infection of biomaterials is a major concern in medicine, and different kinds of antimicrobial biomaterial have been developed to deal with this problem. To test the antimicrobial performance of these biomaterials, the airborne bacterial assay is used, which involves the formation of biohazardous bacterial aerosols. We here describe a new experimental set-up which allows safe handling of such pathogenic aerosols, and standardizes critical parameters of this otherwise intractable and strongly user-dependent assay. With this new method, reproducible, thorough antimicrobial data (number of colony forming units and live-dead-stain) was obtained. Poly(oxonorbornene)-based Synthetic Mimics of Antimicrobial Peptides (SMAMPs) were used as antimicrobial test samples. The assay was able to differentiate even between subtle sample differences, such as different sample thicknesses. With this new set-up, the airborne bacterial assay was thus established as a useful, reliable, and realistic experimental method to simulate the contamination of biomaterials with bacteria, for example in an intraoperative setting.

Indexed as

AerosolsSafetyAnti-Bacterial AgentsBiological AssayBiomimetic MaterialsMicrobial Sensitivity TestsReference StandardsSurface PropertiesAerosolsAnti-Bacterial Agents

Identifiers

PMID25360525
PMCPMC4216082
OpenAlexW2069987116

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.