ArticleBiomedical optics express2014
Longitudinal label-free tracking of cell death dynamics in living engineered human skin tissue with a multimodal microscope.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Quantifying UV-induced photodamage for longitudinal live-cell imaging applications of deep-UV microscopy.Biomedical optics express · 2025Article
- Characterization and identification of cell death dynamics by quantitative phase imaging.Journal of biomedical optics · 2022Article
- Assessing the severity of psoriasis through multivariate analysis of optical images from non-lesional skin.Scientific reports · 2020Article
- Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy.Biomedical optics express · 2019Article
- Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy.Biomedical optics express · 2019Article
- Fluorescence lifetime shifts of NAD(P)H during apoptosis measured by time-resolved flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy.Optica · 2018Article
- Investigating the healing mechanisms of an angiogenesis-promoting topical treatment for diabetic wounds using multimodal microscopy.Journal of biophotonics · 2018Article
- Review of optical coherence tomography in oncology.Journal of biomedical optics · 2017Review
- Quantitative characterization of mechanically indented in vivo human skin in adults and infants using optical coherence tomography.Journal of biomedical optics · 2017Article
- A quantitative framework for the analysis of multimodal optical microscopy images.Quantitative imaging in medicine and surgery · 2017Article
- Label-free in vivo cellular-level detection and imaging of apoptosis.Journal of biophotonics · 2017Article
- Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging.Scientific reports · 2016Article
- Longitudinal in vivo tracking of adverse effects following topical steroid treatment.Experimental dermatology · 2016Article
- Optical coherence tomography-guided laser microsurgery for blood coagulation with continuous-wave laser diode.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
We demonstrate real-time, longitudinal, label-free tracking of apoptotic and necrotic cells in living tissue using a multimodal microscope. The integrated imaging platform combines multi-photon microscopy (MPM, based on two-photon excitation fluorescence), optical coherence microscopy (OCM), and fluorescence lifetime imaging microscopy (FLIM). Three-dimensional (3-D) co-registered images are captured that carry comprehensive information of the sample, including structural, molecular, and metabolic properties, based on light scattering, autofluorescence intensity, and autofluorescence lifetime, respectively. Different cell death processes, namely, apoptosis and necrosis, of keratinocytes from different epidermal layers are longitudinally monitored and investigated. Differentiation of the two cell death processes in a complex living tissue environment is enabled by quantitative image analysis and high-confidence classification processing based on the multidimensional, cross-validating imaging data. These results suggest that despite the limitations of each individual label-free modality, this multimodal imaging approach holds the promise for studies of different cell death processes in living tissue and in vivo organs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.