ArticleBiomedical optics express2014
Correlative multicolor 3D SIM and STORM microscopy.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- DISC-3D: dual-hydrogel system enhances optical imaging and enables correlative mass spectrometry imaging of invading multicellular tumor spheroids.Scientific reports · 2023Article
- Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.The Journal of cell biology · 2023Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Molecular dysregulation of ciliary polycystin-2 channels caused by variants in the TOP domain.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A super-resolution platform for correlative live single-molecule imaging and STED microscopy.Nature methods · 2019Article
- Miro clusters regulate ER-mitochondria contact sites and link cristae organization to the mitochondrial transport machinery.Nature communications · 2019Article
- Registration and Visualization of Correlative Super-Resolution Microscopy Data.Biophysical journal · 2019Article
- Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles.Journal of visualized experiments : JoVE · 2018Article
- Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.Current biology : CB · 2017Article
- Cell-free reconstitution reveals centriole cartwheel assembly mechanisms.Nature communications · 2017Article
- Super-resolution Microscopy - Applications in Plant Cell Research.Frontiers in plant science · 2017Review
- Shedding light on biology of bacterial cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ.Nature communications · 2016Article
- Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations.Scientific reports · 2015Article
- Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei.Journal of experimental botany · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that their combination is a powerful means to transform them into versatile tools for the life sciences. Here, we report the development of a combined SIM and STORM setup that maintains the optimal resolution of both methods and which is coupled to image registration to localize biological structures in 3D using multicolor labeling. We utilized this workflow to determine the localization of Bld12p/CrSAS-6 in purified basal bodies of Chlamydomonas reinhardtii with utmost precision, demonstrating its usefulness for accurate molecular mapping in 3D.
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