ArticleBiomedical optics express2014
Wide field intravital imaging by two-photon-excitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy.Biomedical optics express · 2022Article
- Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination.Nature communications · 2021Article
- Selective plane illumination microscopy (SPIM) with time-domain fluorescence lifetime imaging microscopy (FLIM) for volumetric measurement of cleared mouse brain samples.The Review of scientific instruments · 2018Article
- Nonlinear Absorption Response Correlated to Embedded Ag Nanoparticles in BGO Single Crystal: From Two-Photon to Three-Photon Absorption.Scientific reports · 2018Article
- Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Integrated single- and two-photon light sheet microscopy using accelerating beams.Scientific reports · 2017Article
- Imaging tissue-mimic with light sheet microscopy: A comparative guideline.Scientific reports · 2017Article
- Optimizing ultrafast illumination for multiphoton-excited fluorescence imaging.Biomedical optics express · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digital-scanned light-sheet microscopy (DSLM) illuminates a sample in a plane and captures single-photon-excitation fluorescence images with a camera from a direction perpendicular to the light sheet. This method is potentially useful for observing biological specimens, because image acquisition is relatively fast, resulting in reduction of phototoxicity. However, DSLM cannot be effectively applied to high-scattering materials due to the image blur resulting from thickening of the light sheet by scattered photons. However, two-photon-excitation DSLM (2p-DSLM) enables collection of high-contrast image with near infrared (NIR) excitation. In conventional 2p-DSLM, the minimal excitation volume for two-photon excitation restricts the field of view. In this study, we achieved wide-field 2p-DSLM by using a high-pulse energy fiber laser, and then used this technique to perform intravital imaging of a small model fish species, medaka (Oryzias latipes). Wide fields of view (>700 μm) were achieved by using a low-numerical aperture (NA) objective lens and high-peak energy NIR excitation at 1040 nm. We also performed high-speed imaging at near-video rate and successfully captured the heartbeat movements of a living medaka fish at 20 frames/sec.
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