ArticleCytotechnology2016
A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864).
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- In Vitro and In Vivo Studies of Skin Wound Healing Potential of Nanochitosan Grafted Curcumin and Lycopene in Wistar Albino Rats.Cell biochemistry and biophysics · 2026Article
- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
- Reprocessing seafood waste: challenge to develop aquatic clean meat from fish cells.NPJ science of food · 2022Article
- Establishment and characterization of a cell line derived from fin of the endangered Yangtze sturgeon (Acipenser dabryanus).In vitro cellular & developmental biology. Animal · 2020Article
- Expression Profile Analysis of the Cell Cycle in Diploid and TetraploidFrontiers in genetics · 2020Article
- A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages.Scientific reports · 2018Article
- Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793).In vitro cellular & developmental biology. Animal · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new epithelial cell line, Horabagrus brachysoma fin (HBF), was established from the caudal fin tissue of yellow catfish, H. brachysoma and characterized. This HBF cell line was maintained in Leibovitz's-15 medium supplemented with 15 % fetal bovine serum (FBS) and subcultured more than 62 times over a period of 20 months. The HBF cell line consists predominantly of epithelial cells and is able to grow at temperatures between 20 and 35 °C with an optimum temperature of 28 °C. The growth rate of these cells increased as the proportion of FBS increased from 5 to 20 % at 28 °C with optimum growth at the concentrations of 15 % FBS. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that HBF cell line originated from yellow catfish. The HBF cells showed strong positive reaction to the cytokeratin marker, indicating that it was epithelial in nature. HBF cell line was inoculated with tissue homogenate from juveniles of Sea bass, Lates calcarifer infected with viral nervous necrosis virus (VNNV) and found not susceptible to VNNV. The extracellular products of Vibrio cholerae MTCC 3904 were toxic to the HBF cells. These cells were confirmed for the absence of Mycoplasma sp by PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.