ArticleThe Plant journal : for cell and molecular biology2015
Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants.
Article in The Plant journal : for cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Cytogenomic profiling ofJournal of ginseng research · 2026Article
- TONSOKU prevents the formation of large tandem duplications and restrains ATR-WEE1 checkpoint activation.Nature communications · 2026Article
- Regulation of Pre-rRNA Processing in Plant: Mechanisms, Plasticity, and Developmental Implications.Plants (Basel, Switzerland) · 2026Review
- The histone chaperone CAF-1 prevents homologous recombination-mediated instability of the budding yeast ribosomal DNA during replication-coupled DNA double-strand break repair.Nucleic acids research · 2025Article
- Chromatin dynamics and RNA metabolism are double-edged swords for the maintenance of plant genome integrity.Nature plants · 2024Review
- Extrachromosomal circular DNA and structural variants highlight genome instability in Arabidopsis epigenetic mutants.Nature communications · 2023Article
- Structural variation among assembled genomes facilitates development of rapid and low-cost NOR-linked markers and NOR-telomere junction mapping in Arabidopsis.Plant cell reports · 2023Article
- Quantification of 8-International journal of molecular sciences · 2022Article
- Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats.Nature communications · 2022Article
- G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability.Plant physiology · 2021Article
- The rDNA Loci-Intersections of Replication, Transcription, and Repair Pathways.International journal of molecular sciences · 2021Review
- Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants.Nature communications · 2021Article
- It Is Just a Matter of Time: Balancing Homologous Recombination and Non-homologous End Joining at the rDNA Locus During Meiosis.Frontiers in plant science · 2021Review
- G4 Structures in Control of Replication and Transcription of rRNA Genes.Frontiers in plant science · 2020Article
- DNA methylome and transcriptome landscapes revealed differential characteristics of dioecious flowers in papaya.Horticulture research · 2020Article
- Nuclear Chaperone ASF1 is Required for Gametogenesis in Arabidopsis thaliana.Scientific reports · 2019Article
- Meiotic DNA Repair in the Nucleolus Employs a Nonhomologous End-Joining Mechanism.The Plant cell · 2019Article
- Arabidopsis ATRX Modulates H3.3 Occupancy and Fine-Tunes Gene Expression.The Plant cell · 2017Article
- The Absence of the Arabidopsis Chaperone Complex CAF-1 Produces Mitotic Chromosome Abnormalities and Changes in the Expression Profiles of Genes Involved in DNA Repair.Frontiers in plant science · 2017Article
- Variation of 45S rDNA intergenic spacers in Arabidopsis thaliana.Plant molecular biology · 2016Article
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Authors and funding
7 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis thaliana mutants in FAS1 and FAS2 subunits of chromatin assembly factor 1 (CAF1) show progressive loss of 45S rDNA copies and telomeres. We hypothesized that homology-dependent DNA damage repair (HDR) may contribute to the loss of these repeats in fas mutants. To test this, we generated double mutants by crossing fas mutants with knock-out mutants in RAD51B, one of the Rad51 paralogs of A. thaliana. Our results show that the absence of RAD51B decreases the rate of rDNA loss, confirming the implication of RAD51B-dependent recombination in rDNA loss in the CAF1 mutants. Interestingly, this effect is not observed for telomeric repeat loss, which thus differs from that acting in rDNA loss. Involvement of DNA damage repair in rDNA dynamics in fas mutants is further supported by accumulation of double-stranded breaks (measured as γ-H2AX foci) in 45S rDNA. Occurrence of the foci is not specific for S-phase, and is ATM-independent. While the foci in fas mutants occur both in the transcribed (intranucleolar) and non-transcribed (nucleoplasmic) fraction of rDNA, double fas rad51b mutants show a specific increase in the number of the intranucleolar foci. These results suggest that the repair of double-stranded breaks present in the transcribed rDNA region is RAD51B dependent and that this contributes to rDNA repeat loss in fas mutants, presumably via the single-stranded annealing recombination pathway. Our results also highlight the importance of proper chromatin assembly in the maintenance of genome stability.
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