ArticleScientific reports2014
Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Silk components and properties of the multilayer cocoon of the greater wax moth, Galleria mellonella.Insect science · 2026Article
- Cryo-ET comparison of the hierarchical ultrastructure of silkworm, spider, and artificial silk fibers.Nature communications · 2026Article
- Emerging tools to advance neuroethology in butterflies and moths.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
- Modifying Naturally Occurring, Nonmammalian-Sourced Biopolymers for Biomedical Applications.ACS biomaterials science & engineering · 2024Review
- Overview and Evolution of Insect Fibroin Heavy Chain (FibH).International journal of molecular sciences · 2024Article
- High-Throughput Screening of PAM-Flexible Cas9 Variants for Expanded Genome Editing in the Silkworm (Insects · 2024Article
- High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Genome research · 2024Article
- Evaluating bio-physicochemical properties of raw powder prepared from whole larvae containing liquid silk of the domestic silkworm.Frontiers in nutrition · 2024Article
- Function of Polyamines in Regulating Cell Cycle Progression of Cultured Silkworm Cells.Insects · 2021Article
- Article
- A Targeted In-Fusion Expression System for Recombinant Protein Production inFrontiers in genetics · 2021Article
- Transdermal peptide conjugated to human connective tissue growth factor with enhanced cell proliferation and hyaluronic acid synthesis activities produced by a silkworm silk gland bioreactor.Applied microbiology and biotechnology · 2020Article
- Deep Insight into the Transcriptome of the Single Silk Gland ofInternational journal of molecular sciences · 2019Article
- Mass spider silk production through targeted gene replacement inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Programmable Single and Multiplex Base-Editing inG3 (Bethesda, Md.) · 2018Article
- New insight into the mechanism underlying the silk gland biological process by knocking out fibroin heavy chain in the silkworm.BMC genomics · 2018Article
- QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing.Scientific reports · 2017Article
- Increasing the yield of middle silk gland expression system through transgenic knock-down of endogenous sericin-1.Molecular genetics and genomics : MGG · 2017Article
- Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evolution has produced some remarkable creatures, of which silk gland is a fascinating organ that exists in a variety of insects and almost half of the 34,000 spider species. The impressive ability to secrete huge amount of pure silk protein, and to store proteins at an extremely high concentration (up to 25%) make the silk gland of Bombyx mori hold great promise to be a cost-effective platform for production of recombinant proteins. However, the extremely low production yields of the numerous reported expression systems greatly hindered the exploration and application of silk gland bioreactors. Using customized zinc finger nucleases (ZFN), we successfully performed genome editing of Bmfib-H gene, which encodes the largest and most abundant silk protein, in B. mori with efficiency higher than any previously reported. The resulted Bmfib-H knocked-out B. mori showed a smaller and empty silk gland, abnormally developed posterior silk gland cells, an extremely thin cocoon that contain only sericin proteins, and a slightly heavier pupae. We also showed that removal of endogenous Bmfib-H protein could significantly increase the expression level of exogenous protein. Furthermore, we demonstrated that the bioreactor is suitable for large scale production of protein-based materials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.