ArticleStem cell reports2014
Generation of haploid spermatids with fertilization and development capacity from human spermatogonial stem cells of cryptorchid patients.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 92 citations in OpenAlex.
- Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways.Experimental biology and medicine (Maywood, N.J.) · 2015Trial
- RAD21L1 Is Sufficient and Effective for Reprogramming Human Sertoli Cells to Phenotypic Spermatogonial Stem Cells Through DNA Methylation and Essential for Male Fertility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Novel mutation leading to splice donor loss in a conserved site ofJournal of medical genetics · 2024Article
- Review
- Prior exposure to alkylating agents negatively impacts testicular organoid formation in cells obtained from childhood cancer patients.Human reproduction open · 2024Article
- Current Progress in Stem Cell Therapy for Male Infertility.Stem cell reviews and reports · 2023Review
- Co-Culture of Cryopreserved Healthy Sertoli Cells with Testicular Tissue of Non-Obstructive Azoospermia (NOA) Patients in Culture Media Containing Follicle-Stimulating Hormone (FSH)/Testosterone Has No Advantage in Germ Cell Maturation.Journal of clinical medicine · 2023Article
- GPx3 knockdown inhibits the proliferation and DNA synthesis and enhances the early apoptosis of human spermatogonial stem cells via mediating CXCL10 and cyclin B1.Frontiers in cell and developmental biology · 2023Article
- Alpha-tocopherol enhances spermatogonial stem cell proliferation and restores mouse spermatogenesis by up-regulating BMI1.Frontiers in nutrition · 2023Article
- Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish,Cells · 2022Article
- Regulation of spermatogonial stem cell self-renewal and proliferation in mammals.Histology and histopathology · 2022Review
- Fertility Preservation and Restoration Options for Pre-Pubertal Male Cancer Patients: Current Approaches.Frontiers in endocrinology · 2022Review
- Deleterious variants in TAF7L cause human oligoasthenoteratozoospermia and its impairing histone to protamine exchange inducing reducedFrontiers in endocrinology · 2022Article
- Direct reprogramming of human Sertoli cells into male germline stem cells with the self-renewal and differentiation potentials via overexpressing DAZL/DAZ2/BOULE genes.Stem cell reports · 2021Article
- Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA.Journal of clinical medicine · 2021Review
- Regulation of Human Spermatogenesis.Advances in experimental medicine and biology · 2021Article
- Hsa-miR-100-3p Controls the Proliferation, DNA Synthesis, and Apoptosis of Human Sertoli Cells by Binding to SGK3.Frontiers in cell and developmental biology · 2021Article
- Article
- Stem cell-based therapies for fertility preservation in males: Current status and future prospects.World journal of stem cells · 2020Review
- Approaches and Technologies in Male Fertility Preservation.International journal of molecular sciences · 2020Review
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11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generation of functional spermatids from azoospermia patients is of unusual significance in the treatment of male infertility. Here, we report an efficient approach to obtain human functional spermatids from cryptorchid patients. Spermatogonia remained whereas meiotic germ cells were rare in cryptorchid patients. Expression of numerous markers for meiotic and postmeiotic male germ cells was enhanced in human spermatogonial stem cells (SSCs) of cryptorchidism patients by retinoic acid (RA) and stem cell factor (SCF) treatment. Meiotic spreads and DNA content assays revealed that RA and SCF induced a remarkable increase of SCP3-, MLH1-, and CREST-positive cells and haploid cells. Single-cell RNA sequencing analysis reflected distinct global gene profiles in embryos derived from round spermatids and nuclei of somatic cells. Significantly, haploid spermatids generated from human SSCs of cryptorchid patients possessed fertilization and development capacity. This study thus provides an invaluable source of autologous male gametes for treating male infertility in azoospermia patients.
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