ArticleScientific reports2014
A novel multilayer immunoisolating encapsulation system overcoming protrusion of cells.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Unlocking Transplant Tolerance with Biomaterials.Advanced healthcare materials · 2025Review
- Customizable Hydrogel Coating of ECM-Based Microtissues for Improved Cell Retention and Tissue Integrity.Gels (Basel, Switzerland) · 2024Article
- Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteins.iScience · 2023Article
- An alginate-based encapsulation system for delivery of therapeutic cells to the CNS.RSC advances · 2022Article
- Design Considerations for Macroencapsulation Devices for Stem Cell Derived Islets for the Treatment of Type 1 Diabetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Insulin-Producing Cell Transplantation Platform for Veterinary Practice.Frontiers in veterinary science · 2020Review
- Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells.Journal of biological engineering · 2020Article
- Centrifugal Force-Driven Modular Micronozzle System: Generation of Engineered Alginate Microspheres.Scientific reports · 2019Article
- Polymeric Approaches to Reduce Tissue Responses Against Devices Applied for Islet-Cell Encapsulation.Frontiers in bioengineering and biotechnology · 2019Review
- Structural changes in alginate-based microspheres exposed to in vivo environment as revealed by confocal Raman microscopy.Scientific reports · 2018Article
- Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-L- histidine/alginate microbeads by platelet-rich plasma.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
- The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics.Drug delivery · 2017Article
- Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture.Lab on a chip · 2017Review
- Bioencapsulation technologies in tissue engineering.Journal of applied biomaterials & functional materials · 2016Review
- Magnetoencapsulated human islets xenotransplanted into swine: a comparison of different transplantation sites.Xenotransplantation · 2016Article
- Survival of encapsulated islets: More than a membrane story.World journal of transplantation · 2016Review
- Safety and function of a new pre-vascularized bioartificial pancreas in an allogeneic rat model.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Application of alginate-microencapsulated therapeutic cells is a promising approach for diseases that require a local and constant supply of therapeutic molecules. However most conventional alginate microencapsulation systems are associated with low mechanical stability and protrusion of cells which is associated with higher surface roughness and limits their clinical application. Here we have developed a novel multilayer encapsulation system that prevents cells from protruding from capsules. The system was tested using a therapeutic protein with anti-tumor activity overexpressed in mammalian cells. The cell containing core of the multilayer capsule was formed by flexible alginate, creating a cell sustaining environment. Surrounded by a poly-L-lysine layer the flexible core was enveloped in a high-G alginate matrix that is less flexible and has higher mechanical stability, which does not support cell survival. The cells in the core of the multilayer capsule did not show growth impairment and protein production was normal for periods up to 70 days in vitro. The additional alginate layer also lowered the surface roughness compared to conventional cell containing alginate-PLL capsules. Our system provides a solution for two important, often overlooked phenomena in cell encapsulation: preventing cell protrusion and improving surface roughness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.