ArticleScientific reports2014
Correlative electron and fluorescence microscopy of magnetotactic bacteria in liquid: toward in vivo imaging.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Impact of oxidative stress onmBio · 2026Article
- Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria.Biofilm · 2025Article
- Real-Time Visualization of Calcium Phosphate Formation on Titanium Dioxide Nanoparticles Using Liquid Transmission Electron Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- Exploring Biomineralization Processes Using In Situ Liquid Transmission Electron Microscopy: A Review.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level.Microbiome · 2024Article
- Correlative SIP-FISH-Raman-SEM-NanoSIMS links identity, morphology, biochemistry, and physiology of environmental microbes.ISME communications · 2022Article
- In situ monitoring of exopolymer-dependent Mn mineralization on bacterial surfaces.Science advances · 2020Article
- Correlative ex situ and Liquid-Cell TEM Observation of Bacterial Cell Membrane Damage Induced by Rough Surface Topology.International journal of nanomedicine · 2020Article
- Advances in Cryo-Correlative Light and Electron Microscopy: Applications for Studying Molecular and Cellular Events.The protein journal · 2019Review
- Self-assembling peptides imaged by correlated liquid cell transmission electron microscopy and MALDI-imaging mass spectrometry.Nature communications · 2019Article
- Genome-Based Metabolic Reconstruction of a Novel Uncultivated Freshwater Magnetotactic coccus "Frontiers in microbiology · 2019Article
- Direct Observation of Early Stages of Growth of Multilayered DNA-Templated Au-Pd-Au Core-Shell Nanoparticles in Liquid Phase.Frontiers in bioengineering and biotechnology · 2019Article
- In situ graphene liquid cell-transmission electron microscopy study of insulin secretion in pancreatic islet cells.International journal of nanomedicine · 2019Article
- Direct mineralogical imaging of economic ore and rock samples with multi-modal nonlinear optical microscopy.Scientific reports · 2018Article
- The 2018 correlative microscopy techniques roadmap.Journal of physics D: Applied physics · 2018Review
- The role of electron irradiation history in liquid cell transmission electron microscopy.Science advances · 2018Article
- Off-axis electron holography of bacterial cells and magnetic nanoparticles in liquid.Journal of the Royal Society, Interface · 2017Article
- Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy.Applied and environmental microbiology · 2017Article
- High-resolution imaging of living mammalian cells bound by nanobeads-connected antibodies in a medium using scanning electron-assisted dielectric microscopy.Scientific reports · 2017Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.
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