Evidence map›Paper›PMID 25358432›Full record

ArticleNature communications2014

A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging.

Jiahui Wu, Ahmed S Abdelfattah, Loïs S Miraucourt, Elena Kutsarova, Araya Ruangkittisakul, Hang Zhou, Klaus Ballanyi, Geoffrey Wicks, Mikhail Drobizhev, Aleksander Rebane and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Subcellular Imaging of Neuronal Calcium Handling In Vivo.Journal of visualized experiments : JoVE · 2023
    Article
  6. Article
  7. Article
  8. Fluorescent Indicators For Biological Imaging of Monatomic Ions.Frontiers in cell and developmental biology · 2022
    Review
  9. Review
  10. Application of Fluorescent Proteins for Functional Dissection of theInternational journal of molecular sciences · 2021
    Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jiahui WuDepartment of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Ahmed S AbdelfattahDepartment of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Loïs S MiraucourtDepartment of Neurology and Neurosurgery, Montreal Neurological Institute, Neuroengineering Program, McGill University, Montreal, Quebec, Canada H3A 2B4.
Elena KutsarovaDepartment of Neurology and Neurosurgery, Montreal Neurological Institute, Neuroengineering Program, McGill University, Montreal, Quebec, Canada H3A 2B4.
Araya RuangkittisakulDepartment of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
Hang ZhouDepartment of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Klaus BallanyiDepartment of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
Geoffrey WicksDepartment of Physics, Montana State University, Bozeman, Montana 59717, USA.
Mikhail DrobizhevDepartment of Physics, Montana State University, Bozeman, Montana 59717, USA.
Aleksander Rebane1] Department of Physics, Montana State University, Bozeman, Montana 59717, USA [2] National Institute of Chemical Physics and Biophysics, Tallinn, Estonia 12618.
Edward S RuthazerDepartment of Neurology and Neurosurgery, Montreal Neurological Institute, Neuroengineering Program, McGill University, Montreal, Quebec, Canada H3A 2B4.
Robert E CampbellDepartment of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.

Funding

All-optical molecular voltmeter for measuring electric fields in proteinsR01GM098083 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI REBANE, ALEKSANDER · 2011 to 2014
$961k
CIHR MOP-123514CIHR MOP-126192CIHR MOP-77567NIGMS NIH HHS R01 GM 098083NIGMS NIH HHS R01 GM098083
6 · The paper itself

Abstract

The introduction of calcium ion (Ca(2+)) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca(2+) dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared window (650-1,000 nm) where tissue is most transparent to light. To address this shortcoming, we developed a long Stokes shift RFP-based Ca(2+) indicator, REX-GECO1, with optimal two-photon excitation at <1,000 nm. REX-GECO1 fluoresces at 585 nm when excited at 480 nm or 910 nm by a one- or two-photon process, respectively. We demonstrate that REX-GECO1 can be used as either a ratiometric or intensiometric Ca(2+) indicator in organotypic hippocampal slice cultures (one- and two-photon) and the visual system of albino tadpoles (two-photon). Furthermore, we demonstrate single excitation wavelength two-colour Ca(2+) and glutamate imaging in organotypic cultures.

Indexed as

Optical ImagingProtein EngineeringProtonsAnimalsCalciumHeLa CellsHumansIndicators and ReagentsLuminescent ProteinsMicroscopy, ConfocalMolecular StructureRatsRats, Sprague-DawleyRed Fluorescent ProteinCalciumIndicators and ReagentsLuminescent ProteinsProtonsRed Fluorescent Protein

Identifiers

PMID25358432
PMCPMC4920544

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.