Evidence map›Paper›PMID 25358003›Full record

ArticleMonoclonal antibodies in immunodiagnosis and immunotherapy2014

Monoclonal antibodies against G protein of spring viremia of carp virus.

Peixiao Luo, Xindi Ruan, Qi Zhang, Zeming Li, Min Wang, Xueqin Liu

Abstract read
In one paragraph

Article in Monoclonal antibodies in immunodiagnosis and immunotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Cyprinid viral diseases and vaccine development.Fish & shellfish immunology · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Peixiao Luo1 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University , Wuhan, Hubei, China .
Xindi Ruan
Qi Zhang
Zeming Li
Min Wang
Xueqin Liu

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Part of the G protein (3094-4170 bp) of spring viremia of carp virus (SVCV) was expressed in Escherichia coli and purified by dialysis in our study. Two clones of monoclonal antibodies (MAbs 1H11 and 4B8) against G protein were generated by fusion of mouse myeloma cell line SP2/0 and spleen lymphocytes from part of G protein (3094-4170 bp) immunized mice. The results of ELISA (enzyme-linked immunosorbent assay), IFA (indirect immunofluorescent assay), and Western blot assay further demonstrated the characterizations of the two MAbs. Both 1H11 and 4B8 were specific to SVCV G protein. Ten pairs of synthesized overlapping peptides were used to identify the epitope of the MAbs. The MAbs are useful in the development of SVCV diagnostic methods.

Indexed as

AnimalsAntibodies, MonoclonalAntibody SpecificityCarpsCell LineEnzyme-Linked Immunosorbent AssayEpitopesEscherichia coliFluorescent Antibody Technique, IndirectGTP-Binding ProteinsLymphocytesMiceRenal DialysisSpleenViremiaAntibodies, MonoclonalEpitopesGTP-Binding Proteins

Identifiers

PMID25358003
PMCPMC4216518

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.