ArticlePloS one2014
Targeted sequencing of large genomic regions with CATCH-Seq.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03369821 (Understanding Beta-cell Destruction Through the Study of EXtremely Early-onset Type 1 Diabetes), which is not on this map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Understanding Beta-cell Destruction Through the Study of EXtremely Early-onset Type 1 Diabetes (A Musketeers' Memorandum Study)
Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Cost-effective solutions for high-throughput enzymatic DNA methylation sequencing.PLoS genetics · 2025Article
- guidedNOMe-seq quantifies chromatin states at single allele resolution for hundreds of custom regions in parallel.BMC genomics · 2024Article
- CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element.Nature communications · 2022Article
- TET proteins regulate T cell and iNKT cell lineage specification in a TET2 catalytic dependent manner.Frontiers in immunology · 2022Article
- Exonic Variants in Aging-Related Genes Are Predictive of Phenotypic Aging Status.Frontiers in genetics · 2019Article
- Stage-specific epigenetic regulation of CD4 expression by coordinated enhancer elements during T cell development.Nature communications · 2018Article
- Selective nanopore sequencing of human BRCA1 by Cas9-assisted targeting of chromosome segments (CATCH).Nucleic acids research · 2018Article
- dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization.BMC genomics · 2017Article
- Sequence capture by hybridization to explore modern and ancient genomic diversity in model and nonmodel organisms.Nucleic acids research · 2016Review
- Regulation of DNA methylation dictates Cd4 expression during the development of helper and cytotoxic T cell lineages.Nature immunology · 2015Article
- Recurrent evolution of melanism in South American felids.PLoS genetics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current target enrichment systems for large-scale next-generation sequencing typically require synthetic oligonucleotides used as capture reagents to isolate sequences of interest. The majority of target enrichment reagents are focused on gene coding regions or promoters en masse. Here we introduce development of a customizable targeted capture system using biotinylated RNA probe baits transcribed from sheared bacterial artificial chromosome clone templates that enables capture of large, contiguous blocks of the genome for sequencing applications. This clone adapted template capture hybridization sequencing (CATCH-Seq) procedure can be used to capture both coding and non-coding regions of a gene, and resolve the boundaries of copy number variations within a genomic target site. Furthermore, libraries constructed with methylated adapters prior to solution hybridization also enable targeted bisulfite sequencing. We applied CATCH-Seq to diverse targets ranging in size from 125 kb to 3.5 Mb. Our approach provides a simple and cost effective alternative to other capture platforms because of template-based, enzymatic probe synthesis and the lack of oligonucleotide design costs. Given its similarity in procedure, CATCH-Seq can also be performed in parallel with commercial systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.