ArticleNew microbes and new infections2013
Development of a high throughput PCR to detect Coxiella burnetii and its application in a diagnostic laboratory over a 7-year period.
Article in New microbes and new infections, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Molecular detection ofNew microbes and new infections · 2025Article
- A Comprehensive Review of the Mechanisms of Human Q Fever: Pathogenesis and Pathophysiology.Pathogens (Basel, Switzerland) · 2025Review
- From Culture-Negative to DNA-Positive: The Molecular Revolution in Infective Endocarditis Diagnosis.Pathogens (Basel, Switzerland) · 2025Review
- Real-time PCR detection of Coxiella burnetii in dairy products in Kwara State, Nigeria: a public health concern.BMC veterinary research · 2025Article
- Coxiella burnetii protein CBU2016 supports CCV expansion.Pathogens and disease · 2024Article
- Ticks and Chlamydia-Related Bacteria in Swiss Zoological Gardens Compared to in Contiguous and Distant Control Areas.Microorganisms · 2023Article
- Macrophage infectivity potentiator protein, a peptidyl prolyl cis-trans isomerase, essential for Coxiella burnetii growth and pathogenesis.PLoS pathogens · 2023Article
- Perturbation of ATG16L1 function impairs the biogenesis of Salmonella and Coxiella replication vacuoles.Molecular microbiology · 2022Article
- Metagenomic investigation of potential abortigenic pathogens in foetal tissues from Australian horses.BMC genomics · 2021Article
- Pancreatitis, hypereosinophilia and bilateral pulmonary infiltrates as presentation of acute Q fever.New microbes and new infections · 2021Article
- Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.Molecular & cellular proteomics : MCP · 2021Article
- Article
- Comparison of SARS-CoV-2 RT-PCR on a high-throughput molecular diagnostic platform and the cobas SARS-CoV-2 test for the diagnostic of COVID-19 on various clinical samples.Pathogens and disease · 2020Article
- EirA Is a Novel Protein Essential for Intracellular Replication of Coxiella burnetii.Infection and immunity · 2020Article
- Biogenesis of the SpaciousInfection and immunity · 2020Article
- Article
- Coxiella burnetii utilizes both glutamate and glucose during infection with glucose uptake mediated by multiple transporters.The Biochemical journal · 2019Article
- Prevalence ofNew microbes and new infections · 2019Article
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- Bacterial and protozoal pathogens found in ticks collected from humans in Corum province of Turkey.PLoS neglected tropical diseases · 2018Article
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Q fever is a worldwide zoonotic infectious disease due to Coxiella burnetii. The clinical presentation may be acute (pneumonia and/or hepatitis) or chronic (most commonly endocarditis). Diagnosis mainly relies on serology and PCR. We therefore developed a quantitative real-time PCR. We first tested blindly its performance on various clinical samples and then, when thoroughly validated, we applied it during a 7-year period for the diagnosis of both acute and persistent C. burnetii infection. Analytical sensitivity (< 10 copies/PCR) was excellent. When tested blindly on 183 samples, the specificity of the PCR was 100% (142/142) and the sensitivity was 71% (29/41). The sensitivity was 88% (7/8) on valvular samples, 69% (20/29) on blood samples and 50% (2/4) on urine samples. This new quantitative PCR was then successfully applied for the diagnosis of acute Q fever and endovascular infection due to C. burnetii, allowing the diagnosis of Q fever in six patients over a 7-year period. During a local small cluster of cases, the PCR was also applied to blood from 1355 blood donors; all were negative confirming the high specificity of this test. In conclusion, we developed a highly specific method with excellent sensitivity, which may be used on sera for the diagnosis of acute Q fever and on various samples such as sera, valvular samples, aortic specimens, bone and liver, for the diagnosis of persistent C. burnetii infection.
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