ArticleInternational journal of clinical and experimental medicine2014
GSTP1 arrests bladder cancer T24 cells in G0/G1 phase and up-regulates p21 expression.
Article in International journal of clinical and experimental medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression.Cell death & disease · 2019Article
- High GSTP1 inhibits cell proliferation by reducing Akt phosphorylation and is associated with a better prognosis in hepatocellular carcinoma.Oncotarget · 2018Article
- Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveGSTP1 over-expression was introduced into human bladder cancer T24 cells via the lentivirus system. The influence of GSTP1 on the proliferation and cell cycle of T24 cells as well as the potential mechanisms was investigated.
methodsThe lentiviral vector GSTP1-pWPXL was constructed and transfected into T24 cells in the presence of Lipofectamine 2000. CCK8 assay and colony formation test were performed to explore the impact of GSTP1 on the proliferation of T24 cells. Ollowing PI staining, flow cytometry was done to detect the proportion of T24 cells in different phases. Western blot assay was conducted to detect the protein p21 expression.
resultsWhen compared with control group, T24 cells with GSTP1 over-expression showed significant reduction in cell proliferation (P < 0.01) and they were arrested in G0/G1 phase. Western blot assay indicated that the p21 protein expression in T24 cells with GSTP1 over-expression was significantly higher than that in control group.
conclusionGSTP1 may inhibit the proliferation of T24 cells and arrest these cells in G0/G1 phase, which may be ascribed to the up-regulated expression of p21.
Indexed as
Identifiers
25356172PMC4211822W2340201915What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.