ArticleProceedings of the National Academy of Sciences of the United States of America2014
Building carbon-carbon bonds using a biocatalytic methanol condensation cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 152 citations in OpenAlex.
- Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation.Fundamental research · 2026Article
- Advances in synthetic biology for engineering methylotrophic microbial cell factories.Journal of bacteriology · 2026Review
- Harnessing the potential of microbial methane utilization for chasing sustainability.Current opinion in biotechnology · 2025Review
- A synthetic methylotroph achieves accelerated cell growth by alleviating transcription-replication conflicts.Nature communications · 2025Article
- Electro-Driven Multi-Enzymatic Cascade Conversion of COAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol.Nature communications · 2024Article
- ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA.Nature communications · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- A Light-Driven In Vitro Enzymatic Biosystem for the Synthesis of α-Farnesene from Methanol.Biodesign research · 2024Article
- Reconstruction and metabolic profiling of the genome-scale metabolic network model ofSynthetic and systems biotechnology · 2023Article
- Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives.Synthetic and systems biotechnology · 2023Review
- Metabolic engineering strategies for microbial utilization of methanol.Engineering microbiology · 2023Review
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Enzymatic Conversion of COChemical reviews · 2023Review
- EngineeringBioengineering (Basel, Switzerland) · 2023Article
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- Bioconversion of Methanol by Synthetic Methylotrophy.Advances in biochemical engineering/biotechnology · 2022Article
- Physiological limitations and opportunities in microbial metabolic engineering.Nature reviews. Microbiology · 2022Review
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanol is an important intermediate in the utilization of natural gas for synthesizing other feedstock chemicals. Typically, chemical approaches for building C-C bonds from methanol require high temperature and pressure. Biological conversion of methanol to longer carbon chain compounds is feasible; however, the natural biological pathways for methanol utilization involve carbon dioxide loss or ATP expenditure. Here we demonstrated a biocatalytic pathway, termed the methanol condensation cycle (MCC), by combining the nonoxidative glycolysis with the ribulose monophosphate pathway to convert methanol to higher-chain alcohols or other acetyl-CoA derivatives using enzymatic reactions in a carbon-conserved and ATP-independent system. We investigated the robustness of MCC and identified operational regions. We confirmed that the pathway forms a catalytic cycle through (13)C-carbon labeling. With a cell-free system, we demonstrated the conversion of methanol to ethanol or n-butanol. The high carbon efficiency and low operating temperature are attractive for transforming natural gas-derived methanol to longer-chain liquid fuels and other chemical derivatives.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.