ArticleClinical and vaccine immunology : CVI2015
A eukaryotic expression plasmid carrying chicken interleukin-18 enhances the response to newcastle disease virus vaccine.
Article in Clinical and vaccine immunology : CVI, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Heat Stress Influences Immunity ThroughAnimals : an open access journal from MDPI · 2025Article
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- Co-Formulation of Recombinant Porcine IL-18 Enhances the Onset of Immune Response in a NewVaccines · 2023Article
- Co-administration of chicken IL-2 alleviates clinical signs and replication of the ILTV chicken embryo origin vaccine by pre-activating natural killer cells and cytotoxic T lymphocytes.Journal of virology · 2023Article
- Article
- Colonization of the Gastrointestinal Tract of Chicks with Different Bacterial Microbiota Profiles.Animals : an open access journal from MDPI · 2023Article
- Article
- Current situation and future direction of Newcastle disease vaccines.Veterinary research · 2022Review
- Maternal stevioside supplementation improves intestinal immune function of chicken offspring potentially via modulating gut microbiota and down-regulating the promoter methylation level of suppressor of cytokine signaling 1 (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Article
- Development of PDA Nanoparticles for H9N2 Avian Influenza BPP-V/BP-IV Epitope Peptide Vaccines: Immunogenicity and Delivery Efficiency Improvement.Frontiers in immunology · 2021Article
- AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models.Virology journal · 2020Article
- Recombinant-attenuatedJournal of veterinary science · 2018Article
- Oxidative Stress in Poultry: Lessons from the Viral Infections.Oxidative medicine and cellular longevity · 2018Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin-18 (IL-18) is an important cytokine involved in innate and acquired immunity. In this study, we cloned the full-length chicken IL-18 (ChIL-18) gene from specific-pathogen-free (SPF) chicken embryo spleen cells and provided evidence that the ChIL-18 gene in a recombinant plasmid was successfully expressed in chicken DT40 cells. ChIL-18 significantly enhanced gamma interferon (IFN-γ) mRNA expression in chicken splenocytes, which increased IFN-γ-induced nitric oxide (NO) synthesis by macrophages. The potential genetic adjuvant activity of the ChIL-18 plasmid was examined in chickens by coinjecting ChIL-18 plasmid and inactivated Newcastle disease virus (NDV) vaccine. ChIL-18 markedly elevated serum hemagglutination inhibition (HI) titers and anti-hemagglutinin-neuraminidase (anti-HN)-specific antibody levels, induced the secretion of both Th1- (IFN-γ) and Th2- (interleukin-4) type cytokines, promoted the proliferation of T and B lymphocytes, and increased the populations of CD3(+) T cells and their subsets, CD3(+) CD4(+) and CD3(+) CD8(+) T cells. Furthermore, a virus challenge revealed that ChIL-18 contributed to protection against Newcastle disease virus challenge. Taken together, our data indicate that the coadministration of ChIL-18 plasmid and NDV vaccine induces a strong immune response at both the humoral and cellular levels and that ChIL-18 is a novel immunoadjuvant suitable for NDV vaccination.
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