ArticleNucleic acids research2014
Efficient processing of abasic sites by bacterial nonhomologous end-joining Ku proteins.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Insights into the functional coordination of LigD and Ku in bacterial nonhomologous end joining.Scientific reports · 2026Article
- The Mycobacterium tuberculosis Ku C-terminus is a multi-purpose arm for binding DNA and LigD and stimulating ligation.Nucleic acids research · 2022Article
- The Multifaceted Roles of Ku70/80.International journal of molecular sciences · 2021Review
- Article
- Dynamics of Ku and bacterial non-homologous end-joining characterized using single DNA molecule analysis.Nucleic acids research · 2021Article
- LigD: A Structural Guide to the Multi-Tool of Bacterial Non-Homologous End Joining.Frontiers in molecular biosciences · 2021Review
- Participation of RecJ in the base excision repair pathway of Deinococcus radiodurans.Nucleic acids research · 2020Article
- Bacterial Ligase D preternary-precatalytic complex performs efficient abasic sites processing at double strand breaks during nonhomologous end joining.Nucleic acids research · 2019Article
- Phaeocystis globosa Virus DNA Polymerase X: a "Swiss Army knife", Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair.Scientific reports · 2017Article
- Reactivity and Cross-Linking of 5'-Terminal Abasic Sites within DNA.Chemical research in toxicology · 2017Article
- C-terminal region of bacterial Ku controls DNA bridging, DNA threading and recruitment of DNA ligase D for double strand breaks repair.Nucleic acids research · 2016Article
- Identification of a conserved 5'-dRP lyase activity in bacterial DNA repair ligase D and its potential role in base excision repair.Nucleic acids research · 2016Article
- Multiple and Variable NHEJ-Like Genes Are Involved in Resistance to DNA Damage inFrontiers in microbiology · 2016Article
- Base excision repair enzymes protect abasic sites in duplex DNA from interstrand cross-links.Biochemistry · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular reactive oxygen species as well as the exposure to harsh environmental conditions can cause, in the single chromosome of Bacillus subtilis spores, the formation of apurinic/apyrimidinic (AP) sites and strand breaks whose repair during outgrowth is crucial to guarantee cell viability. Whereas double-stranded breaks are mended by the nonhomologous end joining (NHEJ) system composed of an ATP-dependent DNA Ligase D (LigD) and the DNA-end-binding protein Ku, repair of AP sites would rely on an AP endonuclease or an AP-lyase, a polymerase and a ligase. Here we show that B. subtilis Ku (BsuKu), along with its pivotal role in allowing joining of two broken ends by B. subtilis LigD (BsuLigD), is endowed with an AP/deoxyribose 5'-phosphate (5'-dRP)-lyase activity that can act on ssDNA, nicked molecules and DNA molecules without ends, suggesting a potential role in BER during spore outgrowth. Coordination with BsuLigD makes possible the efficient joining of DNA ends with near terminal abasic sites. The role of this new enzymatic activity of Ku and its potential importance in the NHEJ pathway is discussed. The presence of an AP-lyase activity also in the homolog protein from the distantly related bacterium Pseudomonas aeruginosa allows us to expand our results to other bacterial Ku proteins.
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