Evidence map›Paper›PMID 25355404›Full record

ArticleCell proliferation2014

Icariin attenuates hypoxia-induced oxidative stress and apoptosis in osteoblasts and preserves their osteogenic differentiation potential in vitro.

H-P Ma, X-N Ma, B-F Ge, P Zhen, J Zhou, Y-H Gao, C J Xian, K-M Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 2 pooled it
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 2 syntheses or guidelines pooled it, 63 citations in OpenAlex.

  1. Pooled it
  2. Can Hypoxic Conditioning Improve Bone Metabolism? A Systematic Review.International journal of environmental research and public health · 2019
    Pooled it
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  10. Exercise performance reduction and preventive measures in highland sports.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2023
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  12. Oxidative medicine and cellular longevity · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

H-P MaDepartment of Pharmacy, Lanzhou General Hospital, Lanzhou Command of CPLA, Lanzhou, 730050, China.
X-N Ma
B-F Ge
P Zhen
J Zhou
Y-H Gao
C J Xian
K-M Chen
Lanzhou Army General Hospital · CNUniversity of South Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesIcariin, a prenylated flavonol glycoside isolated from traditional Chinese medicinal herb of the genus Epimedium, has been demonstrated to be a potential alternative therapy for osteoporosis, and its action mechanism so far has been mainly attributed to its phytoestrogenic property. As blood supply to bone is considerably reduced with ageing and by the menopause, we hypothesized that icariin treatment would reduce bone loss by preventing ischaemia-induced hypoxic damages to bone. MATERIALS AND

methodsTo investigate effects of icariin treatment on cultured rat calvarial osteoblasts exposed to hypoxic conditions (2% oxygen).

resultsCompared to normoxic control, cell viability decreased with time to 50% by 48 h in the hypoxic group, and icariin attenuated the reduction, dose dependently, with 10(-6) and 10(-5)  m concentrations showing significant protective effects. Icariin also inhibited increase of lactate dehydrogenase activity in culture media. Measurements on oxidative stress, cell cycling and cell survival indicated that icariin protected osteoblasts by reducing production of reactive oxygen species and malondialdehyde, increasing superoxide dismutase activity, arresting the cell cycle and inhibiting apoptosis. Icariin also preserved osteogenic differentiation potential of the hypoxic cells in a dose-dependent manner, compared to the hypoxia alone group, as revealed by increased levels of RUNX-2, OSX and BMP-2 gene expression, alkaline phosphatase activity, and formation of mineralized nodules.

conclusionsOur results demonstrated that icariin attenuated oxidative stress and apoptosis and preserved viability and osteogenic potential of osteoblasts exposed to hypoxia in vitro, and suggested that its anti-osteoporotic effect may be attributed to its anti-hypoxic activity and phytoestrogenic properties.

Indexed as

Cell HypoxiaAnimalsApoptosisCell Cycle CheckpointsCell DifferentiationCells, CulturedDrugs, Chinese HerbalEpimediumFlavonoidsMalondialdehydeOsteoblastsOsteogenesisOxidative StressProliferating Cell Nuclear AntigenRatsRats, Sprague-DawleyDrugs, Chinese HerbalFlavonoidsicariinMalondialdehydeProliferating Cell Nuclear AntigenReactive Oxygen SpeciesSuperoxide Dismutase

Identifiers

PMID25355404
PMCPMC6496789
OpenAlexW2055395534

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.