ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Archaerhodopsin voltage imaging: synaptic calcium and BK channels stabilize action potential repolarization at the Drosophila neuromuscular junction.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- GABA Receptors and KNeurochemical research · 2024Article
- Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Current Practice in Using Voltage Imaging to Record Fast Neuronal Activity: Successful Examples from Invertebrate to Mammalian Studies.Biosensors · 2023Review
- Excitatory and inhibitory neural dynamics jointly tune motion detection.Current biology : CB · 2022Article
- Article
- Depressed neuromuscular transmission causes weakness in mice lacking BK potassium channels.The Journal of general physiology · 2020Article
- The Frog Motor Nerve Terminal Has Very Brief Action Potentials and Three Electrical Regions Predicted to Differentially Control Transmitter Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Optical Studies of Action Potential Dynamics with hVOS probes.Current opinion in biomedical engineering · 2019Article
- Target-wide Induction and Synapse Type-Specific Robustness of Presynaptic Homeostasis.Current biology : CB · 2019Article
- Article
- Genetically Encoded Voltage Indicators Are Illuminating Subcellular Physiology of the Axon.Frontiers in cellular neuroscience · 2019Article
- Drosophila CaV2 channels harboring human migraine mutations cause synapse hyperexcitability that can be suppressed by inhibition of a Ca2+ store release pathway.PLoS genetics · 2018Article
- Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation.eLife · 2018Article
- A postsynaptic PI3K-cII dependent signaling controller for presynaptic homeostatic plasticity.eLife · 2018Article
- A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation.Cell reports · 2017Article
- Nonreciprocal homeostatic compensation inJournal of neurophysiology · 2017Article
- Resistance to action potential depression of a rat axon terminal in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Survival and growth of C57BL/6J mice lacking the BK channel,Physiological reports · 2017Article
- α2δ-3 Is Required for Rapid Transsynaptic Homeostatic Signaling.Cell reports · 2016Article
- Presynaptic BK channels control transmitter release: physiological relevance and potential therapeutic implications.The Journal of physiology · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The strength and dynamics of synaptic transmission are determined, in part, by the presynaptic action potential (AP) waveform at the nerve terminal. The ion channels that shape the synaptic AP waveform remain essentially unknown for all but a few large synapses amenable to electrophysiological interrogation. The Drosophila neuromuscular junction (NMJ) is a powerful system for studying synaptic biology, but it is not amenable to presynaptic electrophysiology. Here, we demonstrate that Archaerhodopsin can be used to quantitatively image AP waveforms at the Drosophila NMJ without disrupting baseline synaptic transmission or neuromuscular development. It is established that Shaker mutations cause a dramatic increase in neurotransmitter release, suggesting that Shaker is predominantly responsible for AP repolarization. Here we demonstrate that this effect is caused by a concomitant loss of both Shaker and slowpoke (slo) channel activity because of the low extracellular calcium concentrations (0.2-0.5 mM) used typically to assess synaptic transmission in Shaker. In contrast, at physiological extracellular calcium (1.5 mM), the role of Shaker during AP repolarization is limited. We then provide evidence that calcium influx through synaptic CaV2.1 channels and subsequent recruitment of Slo channel activity is important, in concert with Shaker, to ensure proper AP repolarization. Finally, we show that Slo assumes a dominant repolarizing role during repetitive nerve stimulation. During repetitive stimulation, Slo effectively compensates for Shaker channel inactivation, stabilizing AP repolarization and limiting neurotransmitter release. Thus, we have defined an essential role for Slo channels during synaptic AP repolarization and have revised our understanding of Shaker channels at this model synapse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.