Evidence map›Paper›PMID 25354905›Full record

ArticleScientific reports2014

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applied to virus identification.

Adriana Calderaro, Maria-Cristina Arcangeletti, Isabella Rodighiero, Mirko Buttrini, Chiara Gorrini, Federica Motta, Diego Germini, Maria-Cristina Medici, Carlo Chezzi, Flora De Conto

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 124 citations in OpenAlex.

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  9. A review of the literature ofFrontiers in microbiology · 2023
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  15. MALDI-TOF-MS and XAS analysis of complexes formed by metallothionein with mercury and/or selenium.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Adriana CalderaroDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Maria-Cristina ArcangelettiDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Isabella RodighieroDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Mirko ButtriniDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Chiara GorriniDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Federica MottaDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Diego GerminiDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Maria-Cristina MediciDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Carlo ChezziDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
Flora De ContoDepartment of Clinical and Experimental Medicine, Unit of Microbiology and Virology, University of Parma, Italy.
University of Parma · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus detection and/or identification traditionally rely on methods based on cell culture, electron microscopy and antigen or nucleic acid detection. These techniques are good, but often expensive and/or time-consuming; furthermore, they not always lead to virus identification at the species and/or type level. In this study, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) was tested as an innovative tool to identify human polioviruses and to identify specific viral protein biomarkers in infected cells. The results revealed MALDI-TOF MS to be an effective and inexpensive tool for the identification of the three poliovirus serotypes. The method was firstly applied to Sabin reference strains, and then to isolates from different clinical samples, highlighting its value as a time-saving, sensitive and specific technique when compared to the gold standard neutralization assay and casting new light on its possible application to virus detection and/or identification.

Indexed as

AnimalsCell LineEnterovirusHumansPoliovirusReproducibility of ResultsSerogroupSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationViral ProteinsVirusesViral Proteins

Identifiers

PMID25354905
PMCPMC4213803
OpenAlexW2028188059

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.