ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2014
Caffeine exposure alters cardiac gene expression in embryonic cardiomyocytes.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Maternal caffeine consumption during pregnancy and offspring cord blood DNA methylation: an epigenome-wide association study meta-analysis.Epigenomics · 2023Pooled it
- Glucose Co-Treatment Modulates Caffeine-Induced Transcriptomic Alterations During Embryogenesis in Zebrafish (Biology · 2026Article
- Deciphering epigenetic regulation in cardiac developmental toxicity: Mechanisms and implications (Review).Molecular medicine reports · 2026Review
- Characterization of caffeine response regulatory variants in vascular endothelial cells.eLife · 2024Article
- The Epigenetic Effects of Coffee.Molecules (Basel, Switzerland) · 2023Review
- Maternal caffeine intake and DNA methylation in newborn cord blood.The American journal of clinical nutrition · 2022Article
- Impacts of Caffeine during Pregnancy.Trends in endocrinology and metabolism: TEM · 2020Review
- Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats.Toxicology research · 2019Article
- Pioglitazone Induces Cardiomyocyte Apoptosis and Inhibits Cardiomyocyte Hypertrophy Via VEGFR-2 Signaling Pathway.Arquivos brasileiros de cardiologia · 2018Article
- Presence of caffeine reversibly interferes with efficacy of acupuncture-induced analgesia.Scientific reports · 2017Article
- Long-term consequences of disrupting adenosine signaling during embryonic development.Molecular aspects of medicine · 2017Review
- In Utero Caffeine Exposure Induces Transgenerational Effects on the Adult Heart.Scientific reports · 2016Article
- Knockdown of DNA methyltransferase 3a alters gene expression and inhibits function of embryonic cardiomyocytes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Using Zebrafish to Implement a Course-Based Undergraduate Research Experience to Study Teratogenesis in Two Biology Laboratory Courses.Zebrafish · 2016Article
- Caffeine and cardiovascular diseases: critical review of current research.European journal of nutrition · 2016Review
- cAMP induces hypertrophy and alters DNA methylation in HL-1 cardiomyocytes.American journal of physiology. Cell physiology · 2015Article
- Transcriptomic Changes in Zebrafish Embryos and Larvae Following Benzo[a]pyrene Exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies demonstrated that in utero caffeine treatment at embryonic day (E) 8.5 alters DNA methylation patterns, gene expression, and cardiac function in adult mice. To provide insight into the mechanisms, we examined cardiac gene and microRNA (miRNA) expression in cardiomyocytes shortly after exposure to physiologically relevant doses of caffeine. In HL-1 and primary embryonic cardiomyocytes, caffeine treatment for 48 h significantly altered the expression of cardiac structural genes (Myh6, Myh7, Myh7b, Tnni3), hormonal genes (Anp and BnP), cardiac transcription factors (Gata4, Mef2c, Mef2d, Nfatc1), and microRNAs (miRNAs; miR208a, miR208b, miR499). In addition, expressions of these genes were significantly altered in embryonic hearts exposed to in utero caffeine. For in utero experiments, pregnant CD-1 dams were treated with 20-60 mg/kg of caffeine, which resulted in maternal circulation levels of 37.3-65.3 μM 2 h after treatment. RNA sequencing was performed on embryonic ventricles treated with vehicle or 20 mg/kg of caffeine daily from E6.5-9.5. Differential expression (DE) analysis revealed that 124 genes and 849 transcripts were significantly altered, and differential exon usage (DEU) analysis identified 597 exons that were changed in response to prenatal caffeine exposure. Among the DE genes identified by RNA sequencing were several cardiac structural genes and genes that control DNA methylation and histone modification. Pathway analysis revealed that pathways related to cardiovascular development and diseases were significantly affected by caffeine. In addition, global cardiac DNA methylation was reduced in caffeine-treated cardiomyocytes. Collectively, these data demonstrate that caffeine exposure alters gene expression and DNA methylation in embryonic cardiomyocytes.
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