ArticleAngewandte Chemie (International ed. in English)2014
Visualizing the chain-flipping mechanism in fatty-acid biosynthesis.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
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- Structural and Dynamic Insights into Acyl Carrier Protein upon Metal Binding and Acylation Revealed by NMR Spectroscopy and MD Simulations.International journal of molecular sciences · 2025Article
- Manipulation and Structural Activity of AcpM inBiochemistry · 2025Article
- Fluorometric Analysis of Carrier-Protein-Dependent Biosynthesis through a Conformationally Sensitive Solvatochromic Pantetheinamide Probe.ACS chemical biology · 2024Article
- Quantitative Characterization of Chain-Flipping of Acyl Carrier Protein ofJournal of the American Chemical Society · 2024Article
- Substrate Sequestration and Chain Flipping in Human Mitochondrial Acyl Carrier Protein.Biochemistry · 2023Article
- Module-Based Polyketide Synthase Engineering forACS synthetic biology · 2023Review
- Conformational Changes of Acyl Carrier Protein Switch the Chain Length Preference of Acyl-ACP Thioesterase ChFatB2.International journal of molecular sciences · 2023Article
- Enzymology of standalone elongating ketosynthases.Chemical science · 2022Review
- Control of Unsaturation inBiochemistry · 2022Article
- Protein-protein interaction based substrate control in theRSC chemical biology · 2021Article
- Structure and mechanistic analyses of the gating mechanism of elongating ketosynthases.ACS catalysis · 2021Article
- Decoding allosteric regulation by the acyl carrier protein.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Deciphering the Binding Interactions betweenInternational journal of molecular sciences · 2021Article
- Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis.Communications biology · 2021Article
- Activity Mapping the Acyl Carrier Protein: Elongating Ketosynthase Interaction in Fatty Acid Biosynthesis.Biochemistry · 2020Article
- A Single Tool to Monitor Multiple Protein-Protein Interactions of theACS infectious diseases · 2019Article
- Molecular basis for interactions between an acyl carrier protein and a ketosynthase.Nature chemical biology · 2019Article
- Structural characterization of HypX responsible for CO biosynthesis in the maturation of NiFe-hydrogenase.Communications biology · 2019Article
- Trapping the Complex Molecular Machinery of Polyketide and Fatty Acid Synthases with Tunable Silylcyanohydrin Crosslinkers.Angewandte Chemie (International ed. in English) · 2018Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The acyl carrier protein (ACP) from fatty acid synthases sequesters elongating products within its hydrophobic core, but this dynamic mechanism remains poorly understood. We exploited solvatochromic pantetheine probes attached to ACP that fluoresce when sequestered. The addition of a catalytic partner lures the cargo out of the ACP and into the active site of the enzyme, thus enhancing fluorescence to reveal the elusive chain-flipping mechanism. This activity was confirmed by the use of a dual solvatochromic cross-linking probe and solution-phase NMR spectroscopy. The chain-flipping mechanism was visualized by single-molecule fluorescence techniques, thus demonstrating specificity between the Escherichia coli ACP and its ketoacyl synthase catalytic partner KASII.
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