ArticleMetabolites2014
An efficient high throughput metabotyping platform for screening of biomass willows.
Article in Metabolites, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
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Who cites it
10 citing papers in PubMed.
- Breeding Novel Chemistry in Willow: New Hetero Diels-Alder Cyclodimers from Arbusculoidin and Salicortin Suggest Parallel Biosynthetic Pathways.Plants (Basel, Switzerland) · 2024Article
- Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS andFoods (Basel, Switzerland) · 2024Article
- LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation ofMetabolites · 2023Article
- Rapid screening of secondary aromatic metabolites in Populus trichocarpa leaves.Biotechnology for biofuels and bioproducts · 2023Article
- Miyabeacin: A new cyclodimer presents a potential role for willow in cancer therapy.Scientific reports · 2020Article
- Phenylalkanoid Glycosides (Non-Salicinoids) from Wood Chips ofMolecules (Basel, Switzerland) · 2019Article
- Optimizing 1DMetabolomics : Official journal of the Metabolomic Society · 2019Review
- Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia.Data in brief · 2018Article
- Salicin-7-sulfate: A new salicinoid from willow and implications for herbal medicine.Fitoterapia · 2018Article
- Triticum monococcum lines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphid Rhopalosiphum padi.The Annals of applied biology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Future improvement of woody biomass crops such as willow and poplar relies on our ability to select for metabolic traits that sequester more atmospheric carbon into biomass, or into useful products to replace petrochemical streams. We describe the development of metabotyping screens for willow, using combined 1D 1H-NMR-MS. A protocol was developed to overcome 1D 1H-NMR spectral alignment problems caused by variable pH and peak broadening arising from high organic acid levels and metal cations. The outcome was a robust method to allow direct statistical comparison of profiles arising from source (leaf) and sink (stem) tissues allowing data to be normalised to a constant weight of the soluble metabolome. We also describe the analysis of two willow biomass varieties, demonstrating how fingerprints from 1D 1H-NMR-MS vary from the top to the bottom of the plant. Automated extraction of quantitative data of 56 primary and secondary metabolites from 1D 1H-NMR spectra was realised by the construction and application of a Salix metabolite spectral library using the Chenomx software suite. The optimised metabotyping screen in conjunction with automated quantitation will enable high-throughput screening of genetic collections. It also provides genotype and tissue specific data for future modelling of carbon flow in metabolic networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.