ArticleThe Journal of biological chemistry2014
Argonaute proteins affect siRNA levels and accumulation of a novel extrachromosomal DNA from the Dictyostelium retrotransposon DIRS-1.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 9 citations in OpenAlex.
- Evolution of microRNAs in Amoebozoa and implications for the origin of multicellularity.Nucleic acids research · 2024Article
- Eukaryotic life without tQCUG: the role of Elongator-dependent tRNA modifications in Dictyostelium discoideum.Nucleic acids research · 2020Article
- DIRS retrotransposons amplify via linear, single-stranded cDNA intermediates.Nucleic acids research · 2020Article
- sRNAs as possible regulators of retrotransposon activity in Cryptococcus gattii VGII.BMC genomics · 2017Article
- RNase H As Gene Modifier, Driver of Evolution and Antiviral Defense.Frontiers in microbiology · 2017Review
- Retrotransposon Domestication and Control inFrontiers in microbiology · 2017Review
- Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein.PLoS genetics · 2016Article
- A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms.PloS one · 2015Article
- A host factor supports retrotransposition of the TRE5-A population in Dictyostelium cells by suppressing an Argonaute protein.Mobile DNA · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The retrotransposon DIRS-1 is the most abundant retroelement in Dictyostelium discoideum and constitutes the pericentromeric heterochromatin of the six chromosomes in D. discoideum. The vast majority of cellular siRNAs is derived from DIRS-1, suggesting that the element is controlled by RNAi-related mechanisms. We investigated the role of two of the five Argonaute proteins of D. discoideum, AgnA and AgnB, in DIRS-1 silencing. Deletion of agnA resulted in the accumulation of DIRS-1 transcripts, the expression of DIRS-1-encoded proteins, and the loss of most DIRS-1-derived secondary siRNAs. Simultaneously, extrachromosomal single-stranded DIRS-1 DNA accumulated in the cytoplasm of agnA- strains. These DNA molecules appear to be products of reverse transcription and thus could represent intermediate structures before transposition. We further show that transitivity of endogenous siRNAs is impaired in agnA- strains. The deletion of agnB alone had no strong effect on DIRS-1 transposon regulation. However, in agnA-/agnB- double mutant strains strongly reduced accumulation of extrachromosomal DNA compared with the single agnA- strains was observed.
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