ArticleToxicological sciences : an official journal of the Society of Toxicology2015
Synchrotron-based imaging of chromium and γ-H2AX immunostaining in the duodenum following repeated exposure to Cr(VI) in drinking water.
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Article in Toxicological sciences : an official journal of the Society of Toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13citing papers in PubMed
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13 citing papers in PubMed, 40 citations in OpenAlex.
- Crypt and Villus Transcriptomic Responses in Mouse Small Intestine Following Oral Exposure to Hexavalent Chromium.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Hexavalent chromium promotes differential binding of CTCF to its cognate sites in Euchromatin.Epigenetics · 2021Article
- Assessment of Mechanistic Data for Hexavalent Chromium-Induced Rodent Intestinal Cancer Using the Key Characteristics of Carcinogens.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Comparison of Gene Expression Responses in the Small Intestine of Mice Following Exposure to 3 Carcinogens Using the S1500+ Gene Set Informs a Potential Common Adverse Outcome Pathway.Toxicologic pathology · 2019Article
- Mechanisms of Chromium-Induced Toxicity.Current opinion in toxicology · 2019Article
- Mutant Frequency is not Increased in Mice Orally Exposed to Sodium Dichromate.Food safety (Tokyo, Japan) · 2019Article
- Integration of mechanistic and pharmacokinetic information to derive oral reference dose and margin-of-exposure values for hexavalent chromium.Journal of applied toxicology : JAT · 2018Article
- Chromium disrupts chromatin organization and CTCF access to its cognate sites in promoters of differentially expressed genes.Epigenetics · 2018Article
- Comparison of Toxicity and Recovery in the Duodenum of B6C3F1 Mice Following Treatment with Intestinal Carcinogens Captan, Folpet, and Hexavalent Chromium.Toxicologic pathology · 2017Article
- High-Throughput Screening Data Interpretation in the Context of In Vivo Transcriptomic Responses to Oral Cr(VI) Exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Myricetin induces apoptosis via endoplasmic reticulum stress and DNA double-strand breaks in human ovarian cancer cells.Molecular medicine reports · 2016Article
- Reevaluation and Classification of Duodenal Lesions in B6C3F1 Mice and F344 Rats from 4 Studies of Hexavalent Chromium in Drinking Water.Toxicologic pathology · 2016Article
- Oral Chromium Exposure and Toxicity.Current environmental health reports · 2015Review
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Authors and funding
13 authors at 1 institution in 1 country.
Chad M ThompsonToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 cthompson@toxstrategies.com.
Jennifer SeiterToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Mark A ChappellToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Ryan V TapperoToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Deborah M ProctorToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Mina SuhToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Jeffrey C WolfToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Laurie C HawsToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 ToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Rock VitaleToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 ToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 ToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Liz MittalToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Christopher R KirmanToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 ToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 ToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510 ToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc.,
Sean M HaysToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
Mark A HarrisToxStrategies, Inc., Katy, Texas 77494, U.S. Army Engineer Research and Development Center, Vicksburg, Mississippi 39180, Photon Sciences Department, Brookhaven National Laboratory, Upton, New York 11973, ToxStrategies, Inc., Mission Viejo, California 92692, Experimental Pathology Laboratories, Sterling, Virginia 20166, ToxStrategies, Inc., Austin, Texas 78731, Environmental Standards, Inc., Valley Forge, Pennsylvania 19482, Summit Toxicology, LLP, Orange Village, Ohio 44022 and Summit Toxicology, LLP, Allenspark, Colorado 80510.
BioStrategies (United States) · US
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current drinking water standards for chromium are for the combined total of both hexavalent and trivalent chromium (Cr(VI) and Cr(III)). However, recent studies have shown that Cr(III) is not carcinogenic to rodents, whereas mice chronically exposed to high levels of Cr(VI) developed duodenal tumors. These findings may suggest the need for environmental standards specific for Cr(VI). Whether the intestinal tumors arose through a mutagenic or non-mutagenic mode of action (MOA) greatly impacts how drinking water standards for Cr(VI) are derived. Herein, X-ray fluorescence (spectro)microscopy (µ-XRF) was used to image the Cr content in the villus and crypt regions of duodena from B6C3F1 mice exposed to 180 mg/l Cr(VI) in drinking water for 13 weeks. DNA damage was also assessed by γ-H2AX immunostaining. Exposure to Cr(VI) induced villus blunting and crypt hyperplasia in the duodenum--the latter evidenced by lengthening of the crypt compartment by ∼2-fold with a concomitant 1.5-fold increase in the number of crypt enterocytes. γ-H2AX immunostaining was elevated in villi, but not in the crypt compartment. µ-XRF maps revealed mean Cr levels >30 times higher in duodenal villi than crypt regions; mean Cr levels in crypt regions were only slightly above background signal. Despite the presence of Cr and elevated γ-H2AX immunoreactivity in villi, no aberrant foci indicative of transformation were evident. These findings do not support a MOA for intestinal carcinogenesis involving direct Cr-DNA interaction in intestinal stem cells, but rather support a non-mutagenic MOA involving chronic wounding of intestinal villi and crypt cell hyperplasia.
Indexed as
ImmunohistochemistrySynchrotronsAnimalsCell Transformation, NeoplasticChromatesChromiumDNA DamageDuodenal NeoplasmsDuodenumFemaleHistonesHyperplasiaIntestinal AbsorptionIntestinal MucosaMiceMicrospectrophotometryChromatesChromiumchromium hexavalent iongamma-H2AX protein, mouseHistonessodium bichromateWater Pollutants, ChemicalcarcinogenesisCr(VI)duodenumH2AXhexavalent chromiummode of actionsynchrotron
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