ArticleNucleic acids research2014
A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Pooled it
- Induction of antiviral RNA interference by avian coronavirus IBV in PBMCs-Mφ.Cell communication and signaling : CCS · 2026Article
- Dicer is cleaved by the Leader protease encoded by foot-and-mouth disease virus to promote infection in mammalian cells.Science advances · 2025Article
- Virus-derived siRNA: Coronavirus and influenza virus trigger antiviral RNAi immunity in birds.Nucleic acids research · 2025Article
- Insight into the Life Cycle of Enterovirus-A71.Viruses · 2025Review
- In silico approach for the identification of tRNA-derived small non-coding RNAs in SARS-CoV infection.Journal of applied genetics · 2024Article
- Enterovirus evolution reveals the mechanism of an RNA-targeted antiviral and determinants of viral replication.Science advances · 2024Article
- A potential role for SARS-CoV-2 small viral RNAs in targeting host microRNAs and modulating gene expression.Scientific reports · 2022Article
- SARS-CoV-2 causes a significant stress response mediated by small RNAs in the blood of COVID-19 patients.Molecular therapy. Nucleic acids · 2022Article
- SUV39H1 regulates corneal epithelial wound healing via H3K9me3-mediated repression of p27.Eye and vision (London, England) · 2022Article
- Changes of Small Non-coding RNAs by Severe Acute Respiratory Syndrome Coronavirus 2 Infection.Frontiers in molecular biosciences · 2022Article
- The emerging role of miRNAs in the pathogenesis of COVID-19: Protective effects of nutraceutical polyphenolic compounds against SARS-CoV-2 infection.International journal of medical sciences · 2022Review
- SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Roles of host small RNAs in the evolution and host tropism of coronaviruses.Briefings in bioinformatics · 2021Article
- Cocaine Triggers Astrocyte-Mediated Synaptogenesis.Biological psychiatry · 2021Article
- Editorial for "Methods to characterize virus small RNAs and RNA structures".Methods (San Diego, Calif.) · 2020Article
- Functional analyses of mammalian virus 5'UTR-derived, small RNAs that regulate virus translation.Methods (San Diego, Calif.) · 2020Article
- IRES-targeting small molecule inhibits enterovirus 71 replication via allosteric stabilization of a ternary complex.Nature communications · 2020Article
- SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs.American journal of physiology. Lung cellular and molecular physiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The roles of virus-derived small RNAs (vsRNAs) have been studied in plants and insects. However, the generation and function of small RNAs from cytoplasmic RNA viruses in mammalian cells remain unexplored. This study describes four vsRNAs that were detected in enterovirus 71-infected cells using next-generation sequencing and northern blots. Viral infection produced substantial levels (>10(5) copy numbers per cell) of vsRNA1, one of the four vsRNAs. We also demonstrated that Dicer is involved in vsRNA1 generation in infected cells. vsRNA1 overexpression inhibited viral translation and internal ribosomal entry site (IRES) activity in infected cells. Conversely, blocking vsRNA1 enhanced viral yield and viral protein synthesis. We also present evidence that vsRNA1 targets stem-loop II of the viral 5' untranslated region and inhibits the activity of the IRES through this sequence-specific targeting. Our study demonstrates the ability of a cytoplasmic RNA virus to generate functional vsRNA in mammalian cells. In addition, we also demonstrate a potential novel mechanism for a positive-stranded RNA virus to regulate viral translation: generating a vsRNA that targets the IRES.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.