ArticleTissue engineering. Part C, Methods2015
Formation of spatially and geometrically controlled three-dimensional tissues in soft gels by sacrificial micromolding.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Mechanical stress remodelling drives epithelial-mesenchymal transition in the tumour microenvironment.Nature nanotechnology · 2026Article
- Alternatives to animal models in gastroenterology and hepatology research.Frontiers in pharmacology · 2026Review
- MechanoMR microparticle (MbioRxiv : the preprint server for biology · 2025Article
- Human breast tissue engineering in health and disease.EMBO molecular medicine · 2024Review
- Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture.International journal of stem cells · 2022Review
- Emerging Microfluidic and Biosensor Technologies for Improved Cancer Theranostics.Advances in experimental medicine and biology · 2022Article
- Volume-constrained microcontainers enable myoepithelial functional differentiation in highly parallel mammary organoid culture.iScience · 2021Article
- Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines.Cancer cell international · 2021Article
- Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.Scientific reports · 2019Article
- Generation of Cost-Effective Paper-Based Tissue Models through Matrix-Assisted Sacrificial 3D Printing.Nano letters · 2019Article
- Hang on tight: reprogramming the cell with microstructural cues.Biomedical microdevices · 2019Review
- Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models.Frontiers in bioengineering and biotechnology · 2018Review
- Fundamentals of Laser-Based Hydrogel Degradation and Applications in Cell and Tissue Engineering.Advanced healthcare materials · 2017Review
- Dissecting the stem cell niche with organoid models: an engineering-based approach.Development (Cambridge, England) · 2017Review
- Probing the luminal microenvironment of reconstituted epithelial microtissues.Scientific reports · 2016Article
- Fabrication of 3-D Reconstituted Organoid Arrays by DNA-Programmed Assembly of Cells (DPAC).Current protocols in chemical biology · 2016Article
- Coupling between apical tension and basal adhesion allow epithelia to collectively sense and respond to substrate topography over long distances.Integrative biology : quantitative biosciences from nano to macro · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Patterned three-dimensional (3D) cell culture models aim to more accurately represent the in vivo architecture of a tissue for the purposes of testing drugs, studying multicellular biology, or engineering functional tissues. However, patterning 3D multicellular structures within very soft hydrogels (<500 Pa) that mimic the physicochemical environment of many tissues remains a challenge for existing methods. To overcome this challenge, we use a Sacrificial Micromolding technique to temporarily form spatially and geometrically defined 3D cell aggregates in degradable scaffolds before transferring and culturing them in a reconstituted extracellular matrix. Herein, we demonstrate that Sacrificial Micromolding (1) promotes cyst formation and proper polarization of established epithelial cell lines, (2) allows reconstitution of heterotypic cell-cell interactions in multicomponent epithelia, and (3) can be used to control the lumenization-state of epithelial cysts as a function of tissue size. In addition, we discuss the potential of Sacrificial Micromolding as a cell-patterning tool for future studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.