Evidence map›Paper›PMID 25351142›Full record

ArticleMicrobial ecology2015

Metagenomic analysis of the airborne environment in urban spaces.

Nicholas A Be, James B Thissen, Viacheslav Y Fofanov, Jonathan E Allen, Mark Rojas, George Golovko, Yuriy Fofanov, Heather Koshinsky, Crystal J Jaing

Abstract read
In one paragraph

Article in Microbial ecology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nicholas A BePhysical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, 94551, USA.
James B Thissen
Viacheslav Y Fofanov
Jonathan E Allen
Mark Rojas
George Golovko
Yuriy Fofanov
Heather Koshinsky
Crystal J Jaing

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The organisms in aerosol microenvironments, especially densely populated urban areas, are relevant to maintenance of public health and detection of potential epidemic or biothreat agents. To examine aerosolized microorganisms in this environment, we performed sequencing on the material from an urban aerosol surveillance program. Whole metagenome sequencing was applied to DNA extracted from air filters obtained during periods from each of the four seasons. The composition of bacteria, plants, fungi, invertebrates, and viruses demonstrated distinct temporal shifts. Bacillus thuringiensis serovar kurstaki was detected in samples known to be exposed to aerosolized spores, illustrating the potential utility of this approach for identification of intentionally introduced microbial agents. Together, these data demonstrate the temporally dependent metagenomic complexity of urban aerosols and the potential of genomic analytical techniques for biosurveillance and monitoring of threats to public health.

Indexed as

Air MicrobiologyBacillus thuringiensisBacteriaBiomassCitiesDistrict of ColumbiaDNA, BacterialDNA Copy Number VariationsEnvironmental MonitoringFungiMetagenomeMetagenomicsSeasonsSequence AlignmentSequence Analysis, DNADNA, Bacterial

Identifiers

PMID25351142
PMCPMC4312561

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.