ArticlePloS one2014
Identification and expression profiling of microRNAs in the brain, liver and gonads of marine medaka (Oryzias melastigma) and in response to hypoxia.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 77 citations in OpenAlex.
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- MicroRNA qPCR normalization in Nile tilapia (Oreochromis niloticus): Effects of acute cold stress on potential reference targets.Fish physiology and biochemistry · 2023Article
- Dysregulation of Serum MicroRNA after Intracerebral Hemorrhage in Aged Mice.Biomedicines · 2023Article
- Review
- Identification and expression analysis of sex biased miRNAs in chinese hook snout carpFrontiers in genetics · 2022Article
- Small RNA sequencing reveals sex-related miRNAs inFrontiers in genetics · 2022Article
- MicroRNAs May Play an Important Role in Sexual Reversal Process of Chinese Soft-Shelled Turtle,Genes · 2021Article
- Identification of microRNAs in Silver Carp (Animals : an open access journal from MDPI · 2021Article
- Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads.Scientific reports · 2021Article
- MicroRNA regulation in hypoxic environments: differential expression of microRNAs in the liver of largemouth bass (Micropterus salmoides).Fish physiology and biochemistry · 2020Article
- Liver-Specific microRNA Identification in Farmed Carp, Labeo bata (Hamilton, 1822), Fed with Starch Diet Using High-Throughput Sequencing.Marine biotechnology (New York, N.Y.) · 2019Article
- High-throughput sequencing reveals microRNAs in response to heat stress in the head kidney of rainbow trout (Oncorhynchus mykiss).Functional & integrative genomics · 2019Article
- Effects of short-time exposure to atrazine on miRNA expression profiles in the gonad of common carp (Cyprinus carpio).BMC genomics · 2019Article
- Selection of suitable candidate genes for miRNA expression normalization in Yellow River Carp (Cyprinus carpio. var).Scientific reports · 2019Article
- Differential Expression of miRNAs in the Respiratory Tree of the Sea CucumberG3 (Bethesda, Md.) · 2017Article
- Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon.BMC genomics · 2017Article
- Dynamic mRNA and miRNA expression analysis in response to hypoxia and reoxygenation in the blunt snout bream (Megalobrama amblycephala).Scientific reports · 2017Article
- Identification and profiling of Cyprinus carpio microRNAs during ovary differentiation by deep sequencing.BMC genomics · 2017Article
- Dynamics of miRNA transcriptome during gonadal development of zebrafish.Scientific reports · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The marine medaka (Oryzias melastigma) has been increasingly used as a fish model for detecting environmental stresses and chemical contaminants in the marine environment. Recent mammalian studies have shown that environmental stresses can alter the expression profiles of microRNAs (miRNAs), leading to transgenerational effects. Here, we use high-throughput Illumina RNA sequencing (RNA-Seq) for miRNA transcriptome analysis of brain, liver, and gonads from sexually mature male and female marine medaka. A total of 128,883,806 filtered sequence reads were generated from six small RNA libraries, identifying a total of 2,125,663 non-redundant sequences. These sequences were aligned and annotated to known animal miRNAs (miRBase) using the BLAST method. A total of 223 distinct miRNA types were identified, with the greatest number expressed in brain tissue. Our data suggested that 55 miRNA types from 34 families are common to all tested tissues, while some of the miRNAs are tissue-enriched or sex-enriched. Quantitative real-time PCR analysis further demonstrated that let-7a, miR-122, and miR-9-3p were downregulated in hypoxic female medaka, while miR-2184 was specifically upregulated in the testis of hypoxic male fish. This is the first study to identify miRNAs in O. melastigma using small RNA deep sequencing technology. Because miRNA expression is highly conserved between marine medaka and other vertebrates, marine medaka may serve as a good model for studies on the functional roles of miRNAs in hypoxia stress response and signaling in marine fish.
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