ArticleJournal of visualized experiments : JoVE2014
Measuring the osmotic water permeability coefficient (Pf) of spherical cells: isolated plant protoplasts as an example.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Leaf hydraulic maze: Abscisic acid effects on bundle sheath, palisade, and spongy mesophyll conductance.Plant physiology · 2023Article
- Out of the blue: Phototropins of the leaf vascular bundle sheath mediate the regulation of leaf hydraulic conductance by blue light.The Plant cell · 2022Article
- SlERF52 regulates SlTIP1;1 expression to accelerate tomato pedicel abscission.Plant physiology · 2021Article
- Modification of the Expression of the Aquaporin ZmPIP2;5 Affects Water Relations and Plant Growth.Plant physiology · 2020Article
- Sucrose-induced Receptor Kinase 1 is Modulated by an Interacting Kinase with Short Extracellular Domain.Molecular & cellular proteomics : MCP · 2019Article
- Differential gene expression and transport functionality in the bundle sheath versus mesophyll - a potential role in leaf mineral homeostasis.Journal of experimental botany · 2017Article
- To Produce or to Survive: How Plastic Is Your Crop Stress Physiology?Frontiers in plant science · 2017Article
- Article
- Do phosphoinositides regulate membrane water permeability of tobacco protoplasts by enhancing the aquaporin pathway?Planta · 2015Article
- The role of plasma membrane aquaporins in regulating the bundle sheath-mesophyll continuum and leaf hydraulics.Plant physiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying AQP regulation mechanisms is crucial for the understanding of water relations at both the cellular and the whole plant levels. Presented here is a simple and very efficient method for the determination of the osmotic water permeability coefficient (P(f)) in plant protoplasts, applicable in principle also to other spherical cells such as frog oocytes. The first step of the assay is the isolation of protoplasts from the plant tissue of interest by enzymatic digestion into a chamber with an appropriate isotonic solution. The second step consists of an osmotic challenge assay: protoplasts immobilized on the bottom of the chamber are submitted to a constant perfusion starting with an isotonic solution and followed by a hypotonic solution. The cell swelling is video recorded. In the third step, the images are processed offline to yield volume changes, and the time course of the volume changes is correlated with the time course of the change in osmolarity of the chamber perfusion medium, using a curve fitting procedure written in Matlab (the 'PfFit'), to yield P(f).
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