ArticleThe ISME journal2015
Denitrification versus respiratory ammonification: environmental controls of two competing dissimilatory NO3(-)/NO2(-) reduction pathways in Shewanella loihica strain PV-4.
Article in The ISME journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 262 citations in OpenAlex.
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- Soil Nitrogen Mineralization Is Driven by Functional Microbiomes Across a North-South Forest in China.Microorganisms · 2025Article
- Putative promiscuous symbionts in deep-sea corals and crinoids may contribute to nitrogen cycling.Microbiome · 2025Article
- Denitrification is a community trait with partial pathways dominating across microbial genomes and biomes.Nature communications · 2025Article
- Effect of substrate pretreatment onRSC advances · 2025Article
- Nitrogen Recovery through Dissimilatory Nitrate Reduction to Ammonium: Impact of Environmental Factors.ACS omega · 2025Article
- Dissimilatory nitrate reductions in soil Neobacillus and Bacillus strains under aerobic condition.Journal of microbiology (Seoul, Korea) · 2025Article
- Hydrogen-dependent dissimilatory nitrate reduction to ammonium enables growth of Campylobacterota isolates.The ISME journal · 2025Article
- Active bacteria driving N2O mitigation and dissimilatory nitrate reduction to ammonium in ammonia recovery bioreactors.The ISME journal · 2025Article
- Biological and Chemical Processes of Nitrate Reduction and Ferrous Oxidation Mediated byMicroorganisms · 2024Article
- How sensor Amt-like proteins integrate ammonium signals.Science advances · 2024Article
- pH selects for distinct NISME communications · 2024Article
- Implications for nitrogen and sulphur cycles: phylogeny and niche-range ofISME communications · 2024Article
- Diversity and community distribution of soil bacterial in the Yellow River irrigation area of Ningxia, China.PloS one · 2024Article
- Phyloecology of nitrate ammonifiers and their importance relative to denitrifiers in global terrestrial biomes.Nature communications · 2023Article
- The 'microbiome counterattack': Insights on the soil and root-associated microbiome in diverse chickpea and lentil genotypes after an erratic rainfall event.Environmental microbiology reports · 2023Article
- Trichlorobacter ammonificans, a dedicated acetate-dependent ammonifier with a novel module for dissimilatory nitrate reduction to ammonia.The ISME journal · 2023Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Denitrification and respiratory ammonification are two competing, energy-conserving NO3(-)/NO2(-) reduction pathways that have major biogeochemical consequences for N retention, plant growth and climate. Batch and continuous culture experiments using Shewanella loihica strain PV-4, a bacterium possessing both the denitrification and respiratory ammonification pathways, revealed factors that determine NO3(-)/NO2(-) fate. Denitrification dominated at low carbon-to-nitrogen (C/N) ratios (that is, electron donor-limiting growth conditions), whereas ammonium was the predominant product at high C/N ratios (that is, electron acceptor-limiting growth conditions). pH and temperature also affected NO3(-)/NO2(-) fate, and incubation above pH 7.0 and temperatures of 30 °C favored ammonium formation. Reverse-transcriptase real-time quantitative PCR analyses correlated the phenotypic observations with nirK and nosZ transcript abundances that decreased up to 1600-fold and 27-fold, respectively, under conditions favoring respiratory ammonification. Of the two nrfA genes encoded on the strain PV-4 genome, nrfA0844 transcription decreased only when the chemostat reactor received medium with the lowest C/N ratio of 1.5, whereas nrfA0505 transcription occurred at low levels (≤3.4 × 10(-2) transcripts per cell) under all growth conditions. At intermediate C/N ratios, denitrification and respiratory ammonification occurred concomitantly, and both nrfA0844 (5.5 transcripts per cell) and nirK (0.88 transcripts per cell) were transcribed. Recent findings suggest that organisms with both the denitrification and respiratory ammonification pathways are not uncommon in soil and sediment ecosystems, and strain PV-4 offers a tractable experimental system to explore regulation of dissimilatory NO3(-)/NO2(-) reduction pathways.
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