ArticlePloS one2014
Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
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- Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology.Microbial cell factories · 2020Article
- CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits inJournal of biological engineering · 2019Article
- T7 RNA polymerase-driven inducible cell lysis for DNA transfer from Escherichia coli to Bacillus subtilis.Microbial biotechnology · 2017Article
- Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome.Microbial cell factories · 2016Article
- Combining Genes from Multiple Phages for Improved Cell Lysis and DNA Transfer from Escherichia coli to Bacillus subtilis.PloS one · 2016Article
- Flagellar region 3b supports strong expression of integrated DNA and the highest chromosomal integration efficiency of the Escherichia coli flagellar regions.Microbial biotechnology · 2015Article
- Identification and validation of novel chromosomal integration and expression loci in Escherichia coli flagellar region 1.PloS one · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be greatly facilitated by simple and effective approaches to introduce genetic constructs into the E. coli chromosome at well-characterised loci. We combined the Red recombinase system of bacteriophage λ and Isothermal Gibson Assembly for rapid integration of novel DNA constructs into the E. coli chromosome. We identified the flagellar region as a promising region for integration and expression of genetic circuits. We characterised integration and expression at four candidate loci, fliD, fliS, fliT, and fliY, of the E. coli flagellar region 3a. The integration efficiency and expression from the four integrations varied considerably. Integration into fliD and fliS significantly decreased motility, while integration into fliT and fliY had only a minor effect on the motility. None of the integrations had negative effects on the growth of the bacteria. Overall, we found that fliT was the most suitable integration site.
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