Evidence map›Paper›PMID 25349920›Full record

ArticleJournal of visualized experiments : JoVE2014

Immunohistochemical and calcium imaging methods in wholemount rat retina.

Allison Sargoy, Steven Barnes, Nicholas C Brecha, Luis Pérez De Sevilla Müller

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023
    Review
  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Allison SargoyDepartment of Neurobiology, University of California, Los Angeles.
Steven BarnesDepartment of Neurobiology, University of California, Los Angeles; Veterans Administration Greater Los Angeles Healthcare System; Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences, Dalhousie University.
Nicholas C BrechaDepartment of Neurobiology, University of California, Los Angeles; Veterans Administration Greater Los Angeles Healthcare System; Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine, University of California, Los Angeles.
Luis Pérez De Sevilla MüllerDepartment of Neurobiology, University of California, Los Angeles; luisperez@mednet.ucla.edu.
University of California, Los Angeles · USDalhousie University · CAVA Greater Los Angeles Healthcare System · US

Funding

The Role of Striosome Neurons in Mediating Opiod RewardP50DA005010 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KENNEDY, PAMELA · 1987 to 2021
$30.7M
NEUROCHEMICAL PATHWAYS IN THE INNER RETINAR01EY004067 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRECHA, NICHOLAS C. · 1985 to 2019
$9.0M
Vision Science Training ProgramT32EY007026 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Alapakkam P Sampath · 1985 to 2026
$5.7M
Transmitter Release From Mammalian Horizontal Cells.R01EY015573 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRECHA, NICHOLAS C. · 2005 to 2017
$4.9M
Neurochemical Pathways in the Inner RetinaR56EY004067 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRECHA, NICHOLAS C. · 2009 to 2009
$462k
Regulation of Calcium Signaling in Retinal Ganglion Cells after Nerve InjuryI01BX000764 · VA · VA GREATER LOS ANGELES HEALTHCARE SYSTEM · PI BRECHA, NICHOLAS C. · 2011 to 2020
–
BLRD VA I01 BX000764Intramural VA VA999999NEI NIH HHS NIH EY04067NEI NIH HHS R01 EY004067NEI NIH HHS R01 EY015573NEI NIH HHS R56 EY004067NEI NIH HHS T32 EY007026NIDA NIH HHS P50 DA005010
6 · The paper itself

Abstract

In this paper we describe the tools, reagents, and the practical steps that are needed for: 1) successful preparation of wholemount retinas for immunohistochemistry and, 2) calcium imaging for the study of voltage gated calcium channel (VGCC) mediated calcium signaling in retinal ganglion cells. The calcium imaging method we describe circumvents issues concerning non-specific loading of displaced amacrine cells in the ganglion cell layer.

Indexed as

AnimalsAxonsCalciumCalcium ChannelsCalcium SignalingFemaleImmunohistochemistryMaleRatsRats, Sprague-DawleyRetinaRetinal Ganglion CellsCalciumCalcium Channels

Identifiers

PMID25349920
PMCPMC4621789
OpenAlexW2077392147

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.