Evidence map›Paper›PMID 25349457›Full record

ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014

Differential response of epiblast stem cells to Nodal and Activin signalling: a paradigm of early endoderm development in the embryo.

Keren Kaufman-Francis, Hwee Ngee Goh, Yoji Kojima, Joshua B Studdert, Vanessa Jones, Melinda D Power, Emilie Wilkie, Erdahl Teber, David A F Loebel, Patrick P L Tam

Open access · bronzeAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. From pluripotency to differentiation: laying foundations for the body pattern in the mouse embryo.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Keren Kaufman-FrancisEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Hwee Ngee GohEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Yoji KojimaEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia Institute of Integrated Cell-Material Science, Kyoto University, Kyoto 606-8501, Japan.
Joshua B StuddertEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Vanessa JonesEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Melinda D PowerEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Emilie WilkieEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia Bioinformatics Group, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
Erdahl TeberBioinformatics Group, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.
David A F LoebelEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia Sydney Medical School, University of Sydney, Sydney, New South Wales 2008, Australia.
Patrick P L TamEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales 2145, Australia Sydney Medical School, University of Sydney, Sydney, New South Wales 2008, Australia ptam@cmri.org.au.
Children's Medical Research Institute · AUUniversity of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mouse epiblast stem cells (EpiSCs) display temporal differences in the upregulation of Mixl1 expression during the initial steps of in vitro differentiation, which can be correlated with their propensity for endoderm differentiation. EpiSCs that upregulated Mixl1 rapidly during differentiation responded robustly to both Activin A and Nodal in generating foregut endoderm and precursors of pancreatic and hepatic tissues. By contrast, EpiSCs that delayed Mixl1 upregulation responded less effectively to Nodal and showed an overall suboptimal outcome of directed differentiation. The enhancement in endoderm potency in Mixl1-early cells may be accounted for by a rapid exit from the progenitor state and the efficient response to the induction of differentiation by Nodal. EpiSCs that readily differentiate into the endoderm cells are marked by a distinctive expression fingerprint of transforming growth factor (TGF)-β signalling pathway genes and genes related to the endoderm lineage. Nodal appears to elicit responses that are associated with transition to a mesenchymal phenotype, whereas Activin A promotes gene expression associated with maintenance of an epithelial phenotype. We postulate that the formation of definitive endoderm (DE) in embryoid bodies follows a similar process to germ layer formation from the epiblast, requiring an initial de-epithelialization event and subsequent re-epithelialization. Our results show that priming EpiSCs with the appropriate form of TGF-β signalling at the formative phase of endoderm differentiation impacts on the further progression into mature DE-derived lineages, and that this is influenced by the initial characteristics of the cell population. Our study also highlights that Activin A, which is commonly used as an in vitro surrogate for Nodal in differentiation protocols, does not elicit the same downstream effects as Nodal, and therefore may not effectively mimic events that take place in the mouse embryo.

Indexed as

AnimalsCell DifferentiationEmbryonic Stem CellsEndodermGene Expression Regulation, DevelopmentalGerm LayersHomeodomain ProteinsInhibin-beta SubunitsMiceNodal ProteinSignal TransductionTransforming Growth Factor betaHomeodomain Proteinsinhibin beta A subunitInhibin-beta SubunitsMixl1 protein, mouseNodal ProteinNodal protein, mouseTransforming Growth Factor betadirected differentiationendoderm differentiationepiblast stem cellsgastrulationlineage propensityTGF-β signalling

Identifiers

PMID25349457
PMCPMC4216471
OpenAlexW2083012260

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.