ArticleProceedings of the National Academy of Sciences of the United States of America2014
Preservation of viral genomes in 700-y-old caribou feces from a subarctic ice patch.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 133 citations in OpenAlex.
- Viromics approaches for the study of viral diversity and ecology in microbiomes.Nature reviews. Genetics · 2026Review
- Sedimentary ancient DNA as part of a multimethod paleoparasitology approach reveals temporal trends in human parasitic burden in the Roman period.PLoS neglected tropical diseases · 2025Article
- Sequencing RNA from old, dried specimens reveals past viromes and properties of long-surviving RNA.bioRxiv : the preprint server for biology · 2024Article
- Virology-The next fifty years.Cell · 2024Article
- The Expanding Diversity of Viruses from Extreme Environments.International journal of molecular sciences · 2024Review
- Diverse Small Circular DNA Viruses Identified in an American Wigeon Fecal Sample.Microorganisms · 2024Article
- Breaking the Ice: A Review of Phages in Polar Ecosystems.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Novel plant-associated genomoviruses from the Brazilian Cerrado biome.Archives of virology · 2023Article
- Historical RNA expression profiles from the extinct Tasmanian tiger.Genome research · 2023Article
- Recent development in antiviral surfaces: Impact of topography and environmental conditions.Heliyon · 2023Review
- First Insights into the Occurrence of Circular Single-Stranded DNA Genomes in Asian and African Cattle.Animals : an open access journal from MDPI · 2023Article
- An Adagio for Viruses, Played Out on Ancient DNA.Genome biology and evolution · 2023Review
- Novel polyomaviruses identified in fecal samples from four carnivore species.Archives of virology · 2023Article
- Review
- Genomics of Ancient Pathogens: First Advances and Prospects.Biochemistry. Biokhimiia · 2022Review
- Family Genomoviridae: 2021 taxonomy update.Archives of virology · 2021Article
- Identification of the Viral Determinant of Hypovirulence and Host Range in Sclerotiniaceae of a Genomovirus Reconstructed from the Plant Metagenome.Journal of virology · 2021Article
- Glacier ice archives nearly 15,000-year-old microbes and phages.Microbiome · 2021Article
- Resurrection of a Viral Internal Ribosome Entry Site from a 700 Year Old Ancient Northwest Territories Cripavirus.Viruses · 2021Article
- ContigExtender: a new approach to improving de novo sequence assembly for viral metagenomics data.BMC bioinformatics · 2021Article
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Authors and funding
16 authors at 8 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses preserved in ancient materials provide snapshots of past viral diversity and a means to trace viral evolution through time. Here, we use a metagenomics approach to identify filterable and nuclease-resistant nucleic acids preserved in 700-y-old caribou feces frozen in a permanent ice patch. We were able to recover and characterize two viruses in replicated experiments performed in two different laboratories: a small circular DNA viral genome (ancient caribou feces associated virus, or aCFV) and a partial RNA viral genome (Ancient Northwest Territories cripavirus, or aNCV). Phylogenetic analysis identifies aCFV as distantly related to the plant-infecting geminiviruses and the fungi-infecting Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 and aNCV as within the insect-infecting Cripavirus genus. We hypothesize that these viruses originate from plant material ingested by caribou or from flying insects and that their preservation can be attributed to protection within viral capsids maintained at cold temperatures. To investigate the tropism of aCFV, we used the geminiviral reverse genetic system and introduced a multimeric clone into the laboratory model plant Nicotiana benthamiana. Evidence for infectivity came from the detection of viral DNA in newly emerged leaves and the precise excision of the viral genome from the multimeric clones in inoculated leaves. Our findings indicate that viral genomes may in some circumstances be protected from degradation for centuries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.