ArticleJournal of bacteriology2015
The rate of osmotic downshock determines the survival probability of bacterial mechanosensitive channel mutants.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- The diverse gating mechanisms of bacterial MscS-like ion channels.Biochemical Society transactions · 2026Review
- The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.Nature communications · 2026Article
- Osmotic stress triggers fast and reversible PMF collapse in Escherichia coli.Biophysical journal · 2026Article
- MscM uses a novel gating mechanism for bacterial mechanosensitive channels.bioRxiv : the preprint server for biology · 2026Article
- Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI.Nature communications · 2025Article
- Peptidoglycan-outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria.Nature microbiology · 2025Article
- MscS inactivation and recovery are slow voltage-dependent processes sensitive to interactions with lipids.Biophysical journal · 2024Article
- Survival of Icelandic airborne microbes towards simulated atmospheric stress factors.Extremophiles : life under extreme conditions · 2023Article
- Oil-Based Sanitization in Low-Moisture Environments: Delivery of Acetic Acid with Water-in-Oil Emulsions.Microbiology spectrum · 2023Article
- Mechanosensitive channel MscS is critical for termination of the bacterial hypoosmotic permeability response.The Journal of general physiology · 2023Article
- Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products.Microbial cell factories · 2023Article
- How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels.International journal of molecular sciences · 2022Review
- Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel.Nature communications · 2022Article
- Asymmetric effects of amphipathic molecules on mechanosensitive channels.Scientific reports · 2022Article
- Decisive structural elements in water and ion permeation through mechanosensitive channels of large conductance: insights from molecular dynamics simulation.RSC advances · 2022Article
- The rpoS gene confers resistance to low osmolarity conditions in Salmonella enterica serovar Typhi.PloS one · 2022Article
- Phylogenomic Insights into Distribution and Adaptation of Bdellovibrionota in Marine Waters.Microorganisms · 2021Article
- Understanding Beta-Lactam-Induced Lysis at the Single-Cell Level.Frontiers in microbiology · 2021Article
- Dual Regulation of Phosphatidylserine Decarboxylase Expression by Envelope Stress Responses.Frontiers in molecular biosciences · 2021Article
- Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1.Channels (Austin, Tex.) · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Mechanosensitive (MS) channels allow cells to sense and respond to environmental changes. In bacteria, these channels are believed to protect against an osmotic shock. The physiological function of these channels has been characterized primarily by a standardized assay, where aliquots of batch-cultured cells are rapidly pipetted into a hypotonic medium. Under this method, it has been inferred many types of MS channels (MscS homologs in Escherichia coli) demonstrate limited effectiveness against shock, typically rescuing less than 10% of the cells when expressed at native levels. We introduce a single-cell-based assay which allows us to control how fast the osmolarity changes, over time scales ranging from a fraction of a second to several minutes. We find that the protection provided by MS channels depends strongly on the rate of osmotic change, revealing that, under a slow enough osmotic drop, MscS homologs can lead to survival rates comparable to those found in wild-type strains. Further, after the osmotic downshift, we observe multiple death phenotypes, which are inconsistent with the prevailing paradigm of how cells lyse. Both of these findings require a reevaluation of our basic understanding of the physiology of MS channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.