ArticleMolecular and cellular biology2015
A novel role for protein kinase Kin2 in regulating HAC1 mRNA translocation, splicing, and translation.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Nitrogen limitation causes a seismic shift in redox state and phosphorylation of proteins implicated in carbon flux and lipidome remodeling in Rhodotorula toruloides.Biotechnology for biofuels and bioproducts · 2025Article
- Crucial roles of Grr1 in splicing and translation of HAC1 mRNA upon unfolded stress response.Nature communications · 2025Article
- The Sequential Recruitments of Rab-GTPase Ypt1p and the NNS Complex onto pre-Molecular and cellular biology · 2023Article
- Determination of the Stability and Intracellular (Intra-Nuclear) Targeting and Recruitment of Pre-HAC1 mRNA in the Saccharomyces cerevisiae During the Activation of UPR.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Vps34 and TOR Kinases CoordinateMolecular and cellular biology · 2021Article
- Phosphorylation of Pal2 by the protein kinases Kin1 and Kin2 modulatesScience signaling · 2021Article
- Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase.Cells · 2019Article
- Translation Control ofInternational journal of molecular sciences · 2019Review
- Adaptation to Endoplasmic Reticulum Stress Requires Transphosphorylation within the Activation Loop of Protein Kinases Kin1 and Kin2, Orthologs of Human Microtubule Affinity-Regulating Kinase.Molecular and cellular biology · 2018Article
- Substrate priming enhances phosphorylation by the budding yeast kinases Kin1 and Kin2.The Journal of biological chemistry · 2018Article
- Identification of stress responsive genes by studying specific relationships between mRNA and protein abundance.Heliyon · 2018Article
- Nuclear mRNA degradation tunes the gain of the unfolded protein response in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- Article
- Evidence That Base-pairing Interaction between Intron and mRNA Leader Sequences Inhibits Initiation of HAC1 mRNA Translation in Yeast.The Journal of biological chemistry · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
A signaling network called the unfolded protein response (UPR) resolves the protein-folding defects in the endoplasmic reticulum (ER) from yeasts to humans. In the yeast Saccharomyces cerevisiae, the UPR activation involves (i) aggregation of the ER-resident kinase/RNase Ire1 to form an Ire1 focus, (ii) targeting HAC1 pre-mRNA toward the Ire1 focus that cleaves out an inhibitory intron from the mRNA, and (iii) translation of Hac1 protein from the spliced mRNA. Targeting HAC1 mRNA to the Ire1 focus requires a cis-acting bipartite element (3'BE) located at the 3' untranslated leader. Here, we report that the 3'BE plays an additional role in promoting translation from the spliced mRNA. We also report that a high dose of either of two paralogue kinases, Kin1 and Kin2, overcomes the defective UPR caused by a mutation in the 3'BE. These results define a novel role for Kin kinases in the UPR beyond their role in cell polarity and exocytosis. Consistently, targeting, splicing, and translation of HAC1 mRNA are substantially reduced in the kin1Δ kin2Δ strain. Furthermore, we show that Kin2 kinase domain itself is sufficient to activate the UPR, suggesting that Kin2 initiates a signaling cascade to ensure an optimum UPR.
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