ArticleInfection and immunity2015
Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
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- Occupational health of drilling waste workers as related to microbial exposure and waste treatment methods.Annals of work exposures and health · 2025Article
- Bioaerosol-induced in vitro activation of toll-like receptors and inflammatory biomarker expression in waste workers.International archives of occupational and environmental health · 2023Article
- Divergent TLR2 and TLR4 Activation by Fungal Spores and Species Diversity in Dust from Waste Sorting Plants.Applied and environmental microbiology · 2023Article
- Bioaerosol Exposure andSafety and health at work · 2022Article
- Dose-Sparing Intradermal DTaP-sIPV Immunization With a Hollow Microneedle Leads to Superior Immune Responses.Frontiers in microbiology · 2021Article
- Shortening the Lipid A Acyl Chains ofVaccines · 2020Article
- Substrate specificity of the pyrophosphohydrolase LpxH determines the asymmetry ofThe Journal of biological chemistry · 2019Article
- Activation of Human NK Cells byFrontiers in immunology · 2019Article
- Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination.Mucosal immunology · 2018Article
- Bordetella pertussis pertactin knock-out strains reveal immunomodulatory properties of this virulence factor.Emerging microbes & infections · 2018Article
- Virulence Associated Gene 8 ofFrontiers in immunology · 2018Article
- Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bordetella pertussis is a Gram-negative bacterium and the causative agent of whooping cough. Despite high vaccination coverage, outbreaks are being increasingly reported worldwide. Possible explanations include adaptation of this pathogen, which may interfere with recognition by the innate immune system. Here, we describe innate immune recognition and responses to different B. pertussis clinical isolates. By using HEK-Blue cells transfected with different pattern recognition receptors, we found that 3 out of 19 clinical isolates failed to activate Toll-like receptor 4 (TLR4). These findings were confirmed by using the monocytic MM6 cell line. Although incubation with high concentrations of these 3 strains resulted in significant activation of the MM6 cells, it was found to occur mainly through interaction with TLR2 and not through TLR4. When using live bacteria, these 3 strains also failed to activate TLR4 on HEK-Blue cells, and activation of MM6 cells or human monocyte-derived dendritic cells was significantly lower than activation induced by the other 16 strains. Mass spectrum analysis of the lipid A moieties from these 3 strains indicated an altered structure of this molecule. Gene sequence analysis revealed mutations in genes involved in lipid A synthesis. Findings from this study indicate that B. pertussis isolates that do not activate TLR4 occur naturally and that this phenotype may give this bacterium an advantage in tempering the innate immune response and establishing infection. Knowledge on the strategies used by this pathogen in evading the host immune response is essential for the improvement of current vaccines or for the development of new ones.
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