ArticlePhysiological reports2014
Low oxygen tension enhances the generation of lung progenitor cells from mouse embryonic and induced pluripotent stem cells.
Article in Physiological reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- The mechanical microenvironment and lung stem cell fate.Frontiers in cell and developmental biology · 2026Review
- Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs.International journal of molecular sciences · 2022Article
- Induced pluripotent stem cell-based disease modeling and prospective immune therapy for coronavirus disease 2019.Cytotherapy · 2022Review
- Mouse Pluripotent Stem Cell Differentiation Under Physiological Oxygen Reduces Residual Teratomas.Cellular and molecular bioengineering · 2021Article
- Oxygen Biosensors and Control in 3D Physiomimetic Experimental Models.Antioxidants (Basel, Switzerland) · 2021Review
- Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration.Stem cell research & therapy · 2020Article
- Lung Microvascular Niche, Repair, and Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- A Translational Model of Incomplete Catch-Up Growth: Early-Life Hypoxia and the Effect of Physical Activity.Clinical and translational science · 2018Article
- Acellular human lung scaffolds to model lung disease and tissue regeneration.European respiratory review : an official journal of the European Respiratory Society · 2018Review
- Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds.Scientific reports · 2017Article
- Physiological oxygen tension reduces hepatocyte dedifferentiation in in vitro culture.Scientific reports · 2017Article
- Advances in the Knowledge about Kidney Decellularization and Repopulation.Frontiers in bioengineering and biotechnology · 2017Review
- Lung bioengineering: physical stimuli and stem/progenitor cell biology interplay towards biofabricating a functional organ.Respiratory research · 2016Review
- Nuclear Factor κB1/RelA Mediates Inflammation in Human Lung Epithelial Cells at Atmospheric Oxygen Levels.Journal of cellular physiology · 2016Article
- Hypoxia increases genome-wide bivalent epigenetic marking by specific gain of H3K27me3.Epigenetics & chromatin · 2016Article
- A Novel Chip for Cyclic Stretch and Intermittent Hypoxia Cell Exposures Mimicking Obstructive Sleep Apnea.Frontiers in physiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-organ decellularization technology has emerged as a new alternative for the fabrication of bioartificial lungs. Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) are potentially useful for recellularization since they can be directed to express phenotypic marker genes of lung epithelial cells. Normal pulmonary development takes place in a low oxygen environment ranging from 1 to 5%. By contrast, in vitro ESC and iPSC differentiation protocols are usually carried out at room-air oxygen tension. Here, we sought to determine the role played by oxygen tension on the derivation of Nkx2.1+ lung/thyroid progenitor cells from mouse ESC and iPSC. A step-wise differentiation protocol was used to generate Nkx2.1+ lung/thyroid progenitors under 20% and 5% oxygen tension. On day 12, gene expression analysis revealed that Nkx2.1 and Foxa2 (endodermal and early lung epithelial cell marker) were significantly upregulated at 5% oxygen tension in ESC and iPSC differentiated cultures compared to 20% oxygen conditions. In addition, quantification of Foxa2+Nkx2.1+Pax8- cells corresponding to the lung field, with exclusion of the potential thyroid fate identified by Pax8 expression, confirmed that the low physiologic oxygen tension exerted a significant positive effect on early pulmonary differentiation of ESC and iPSC. In conclusion, we found that 5% oxygen tension enhanced the derivation of lung progenitors from mouse ESC and iPSC compared to 20% room-air oxygen tension.
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