Evidence map›Paper›PMID 25347742›Full record

ArticleOncogene2015

EBV-miR-BART7-3p promotes the EMT and metastasis of nasopharyngeal carcinoma cells by suppressing the tumor suppressor PTEN.

L-M Cai, X-M Lyu, W-R Luo, X-F Cui, Y-F Ye, C-C Yuan, Q-X Peng, D-H Wu, T-F Liu, E Wang and 5 more

Abstract read
PubMed Publisher
In one paragraph

Article in Oncogene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
132citing papers in PubMed, 1 pooled it
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

132 citing papers in PubMed, 1 synthesis or guideline pooled it, 195 citations in OpenAlex.

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72 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

L-M CaiCancer Research Institute, Southern Medical University, Guangzhou, China.
X-M Lyu1] Cancer Research Institute, Southern Medical University, Guangzhou, China [2] Department of Laboratory Medicine, the Third Affiliated Hospital, Southern Medical University, Guangzhou, China.
W-R LuoCancer Research Institute, Southern Medical University, Guangzhou, China.
X-F CuiDepartment of ENT, 463 Hospital of the Chinese PLA, Shenyang, China.
Y-F YeCancer Research Institute, Southern Medical University, Guangzhou, China.
C-C YuanCancer Research Institute, Southern Medical University, Guangzhou, China.
Q-X PengSchool of Chinese Traditional Medicine, Southern Medical University, Guangzhou, China.
D-H WuDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
T-F LiuDepartment of Pathology, Southern Medical University, Guangzhou, China.
E WangInfectious Diseases and Immunogenetics Section, DTM, Clinical Center, the National Institutes of Health, Bethesda, MD, USA.
F-M MarincolaInfectious Diseases and Immunogenetics Section, DTM, Clinical Center, the National Institutes of Health, Bethesda, MD, USA.
K-T YaoCancer Research Institute, Southern Medical University, Guangzhou, China.
W-Y FangCancer Research Institute, Southern Medical University, Guangzhou, China.
H-B CaiSchool of Chinese Traditional Medicine, Southern Medical University, Guangzhou, China.
X LiCancer Research Institute, Southern Medical University, Guangzhou, China.
Southern Medical University · CNNational Institutes of Health Clinical Center · USNanfang Hospital · CNShenyang 242 Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The epithelial-mesenchymal transition (EMT) is crucial to cancer progression and metastasis. Although multiple cellular miRNAs have been identified to regulate the EMT and metastasis in cancers, the role of viral miRNAs in cancer progression remains largely unknown. Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy typically characterized by its early metastasis. In the present study, we have discovered the involvement of a viral miRNA, EBV-miR-BART7-3p, in the EMT and metastasis of NPC cells. Initially, we observed that EBV-miR-BART7-3p was highly expressed in NPC and positively correlated with lymph node metastasis and clinical stage of NPC. Subsequently, we demonstrated that EBV-miR-BART7-3p enhanced cell migration/invasion in vitro, cancer metastasis in vivo, and particularly the EMT characterized by loss of epithelial markers and gain of mesenchymal features in NPC cells. Furthermore, mechanistic studies disclosed that EBV-miR-BART7-3p targeted a major human tumor suppressor PTEN, modulating PI3K/Akt/GSK-3β signaling and eventually leading to the high expression and nuclear accumulation of Snail and β-catenin, which favor EMT. Knockdown of PTEN could phenocopy the effect of EBV-miR-BART7-3p, whereas re-expression of PTEN resulted in a phenotypic reversion. Moreover, these findings were supported by an observation of an EBV-positive cell model in which silencing of endogenous EBV-miR-BART7-3p partially attenuated cell migration/invasion and altered EMT protein expression pattern via reverting PI3K/Akt, Snail and β-catenin expression. Thus, this study suggests a novel mechanism by which EBV-miR-BART7-3p modulates the EMT and metastasis of NPC cells, and a clinical implication of EBV-miR-BART7-3p as a potential biomarker or therapeutic target.

Indexed as

Epithelial-Mesenchymal TransitionCarcinomaCell LineCell NucleusEpstein-Barr Virus InfectionsFemaleGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHerpesvirus 4, HumanHumansNasopharyngeal CarcinomaNasopharyngeal NeoplasmsNeoplasm InvasivenessNeoplasm MetastasisPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaGSK3B protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktPTEN PhosphohydrolasePTEN protein, humanRNA, NeoplasmRNA, Viral

Identifiers

PMID25347742
OpenAlexW2045361386

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.