ArticleAnalytical chemistry2014
Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Accelerated protein digestion by numbering-up nanochannels, followed by LC-MS analysis.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Nested Nanowell Arrays for High-Throughput Quantitative Analysis of Cytokines from Single Macrophages.ACS nano medicine · 2026Article
- From Single Cells to Silicon: Emerging Technologies Transforming Monoclonal Antibody Discovery.Antibodies (Basel, Switzerland) · 2026Review
- Synthetic Cell-Based Artificial Stem Cell Niches for Hematopoietic Stem Cell Differentiation.Chembiochem : a European journal of chemical biology · 2026Article
- Multiplexed profiling of single-cell secretion on a hierarchical loading microwell chip.Biosensors & bioelectronics · 2025Article
- Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells.Nature communications · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Quantitative live-cell imaging of secretion activity reveals dynamic immune responses.iScience · 2024Article
- Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting.Nature biomedical engineering · 2024Article
- Antibodies, repertoires and microdevices in antibody discovery and characterization.Lab on a chip · 2024Review
- Digital PCR for Single-Cell Analysis.Biosensors · 2024Review
- Probing T-cell activation in nanoliter tumor co-cultures using membrane displacement trap arrays.Integrative biology : quantitative biosciences from nano to macro · 2024Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Review
- Single-cell sorting based on secreted products for functionally defined cell therapies.Microsystems & nanoengineering · 2022Review
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Review
- Single-Cell Technologies for the Study of Antibody-Secreting Cells.Frontiers in immunology · 2021Review
- Circulating Metabolites as Potential Biomarkers for Neurological Disorders-Metabolites in Neurological Disorders.Metabolites · 2020Article
- Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip.Cell reports · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Arrays of subnanoliter wells (nanowells) provide a useful system to isolate single cells and analyze their secreted proteins. Two general approaches have emerged: one that uses open arrays and local capture of secreted proteins, and a second (called microengraving) that relies on closed arrays to capture secreted proteins on a solid substrate, which is subsequently removed from the array. However, the design and operating parameters for efficient capture from these two approaches to analyze single-cell secretion have not been extensively considered. Using numerical simulations, we analyzed the operational envelope for both open and closed formats, as a function of the spatial distribution of capture ligands, their affinities for the protein, and the rates of single-cell secretion. Based on these analyses, we present a modified approach to capture secreted proteins in-well for highly active secreting cells. This simple method for in-well detection should facilitate rapid identification of cell lines with high specific productivities.
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Registered trials
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