ArticleStem cells (Dayton, Ohio)2015
Neural crest specification by inhibition of the ROCK/Myosin II pathway.
Article in Stem cells (Dayton, Ohio), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Conservation of Epithelial-to-Mesenchymal Transition Process in Neural Crest Cells and Metastatic Cancer.Cells, tissues, organs · 2021Pooled it
- Transposable Element Dynamics Drive the Genomic Evolution and Phenotypic Diversification of Allotetraploid Common Carp.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Extracellular presentation of syntaxin4 as a potential trigger for region-specific gastrulation.Cell structure and function · 2025Article
- NEUROD1 efficiently converts peripheral blood cells into neurons with partial reprogramming by pluripotency factors.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Pathogenic variants in SHROOM3 associated with hemifacial microsomia.Journal of human genetics · 2025Article
- Digital PCR-Based Gene Expression Analysis Using a Highly Multiplexed Assay with Universal Detection Probes to Study Induced Pluripotent Stem Cell Differentiation into Cranial Neural Crest Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Rho-Associated Protein Kinase Activity Is Required for Tissue Homeostasis in theJournal of developmental biology · 2024Article
- Mechanically guided cell fate determination in early development.Cellular and molecular life sciences : CMLS · 2024Review
- Generation of human elongating multi-lineage organized cardiac gastruloids.STAR protocols · 2022Article
- Rostrocaudal patterning and neural crest differentiation of human pre-neural spinal cord progenitors in vitro.Stem cell reports · 2022Article
- Review
- Nonmuscle Myosin II is Required for Larval Shell Formation in a Patellogastropod.Frontiers in cell and developmental biology · 2022Article
- Fine-tuning of dual-SMAD inhibition to differentiate human pluripotent stem cells into neural crest stem cells.Cell proliferation · 2021Article
- Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons.Frontiers in cellular neuroscience · 2020Article
- ROCK inhibitor increases proacinar cells in adult salivary gland organoids.Stem cell research · 2019Article
- Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences.Stem cells translational medicine · 2019Article
- Dynamic blebbing: A bottleneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling.Stem cell research · 2018Article
- ROCK-nmMyoII, Notch andDevelopment (Cambridge, England) · 2018Article
- Regulation of neural crest development by the formin family protein Daam1.Genesis (New York, N.Y. : 2000) · 2018Article
- The Rho-associated kinase inhibitors Y27632 and fasudil promote microglial migration in the spinal cord via the ERK signaling pathway.Neural regeneration research · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neural crest is a population of multipotent progenitor cells that form at the border of neural and non-neural ectoderm in vertebrate embryos, and undergo epithelial-mesenchymal transition and migration. According to the traditional view, the neural crest is specified in early embryos by signaling molecules including BMP, FGF, and Wnt proteins. Here, we identify a novel signaling pathway leading to neural crest specification, which involves Rho-associated kinase (ROCK) and its downstream target nonmuscle Myosin II. We show that ROCK inhibitors promote differentiation of human embryonic stem cells (hESCs) into neural crest-like progenitors (NCPs) that are characterized by specific molecular markers and ability to differentiate into multiple cell types, including neurons, chondrocytes, osteocytes, and smooth muscle cells. Moreover, inhibition of Myosin II was sufficient for generating NCPs at high efficiency. Whereas Myosin II has been previously implicated in the self-renewal and survival of hESCs, we demonstrate its role in neural crest development during ESC differentiation. Inhibition of this pathway in Xenopus embryos expanded neural crest in vivo, further indicating that neural crest specification is controlled by ROCK-dependent Myosin II activity. We propose that changes in cell morphology in response to ROCK and Myosin II inhibition initiate mechanical signaling leading to neural crest fates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.