ArticleCellular microbiology2015
Opa+ Neisseria gonorrhoeae exhibits reduced survival in human neutrophils via Src family kinase-mediated bacterial trafficking into mature phagolysosomes.
Article in Cellular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 34 citations in OpenAlex.
- Neutrophil CEACAMs and the Inflammatory Response toJournal of innate immunity · 2026Review
- Article
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- Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils.Journal of leukocyte biology · 2023Article
- Neisseria gonorrhoeae co-opts C4b-binding protein to enhance complement-independent survival from neutrophils.PLoS pathogens · 2023Article
- Variable Expression of Opa Proteins by Neisseria gonorrhoeae Influences Bacterial Association and Phagocytic Killing by Human Neutrophils.Journal of bacteriology · 2022Article
- Tissue Models forFrontiers in cellular and infection microbiology · 2022Review
- Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages.PLoS pathogens · 2021Article
- Manipulating neutrophil degranulation as a bacterial virulence strategy.PLoS pathogens · 2020Review
- The HopQ-CEACAM Interaction Controls CagA Translocation, Phosphorylation, and Phagocytosis ofmBio · 2020Article
- Cathepsins in Bacteria-Macrophage Interaction: Defenders or Victims of Circumstance?Frontiers in cellular and infection microbiology · 2020Review
- Whole Transcriptome Analysis Reveals ThatFrontiers in immunology · 2020Article
- Cell intrinsic functions of neutrophils and their manipulation by pathogens.Current opinion in immunology · 2019Review
- Complement alone drives efficacy of a chimeric antigonococcal monoclonal antibody.PLoS biology · 2019Article
- Progress Toward a Gonococcal Vaccine: The Way Forward.Frontiers in immunology · 2019Review
- Protocols to Interrogate the Interactions Between Neisseria gonorrhoeae and Primary Human Neutrophils.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Gonococcal Defenses against Antimicrobial Activities of Neutrophils.Trends in microbiology · 2018Review
- Specific Binding to Differentially Expressed Human Carcinoembryonic Antigen-Related Cell Adhesion Molecules Determines the Outcome of Neisseria gonorrhoeae Infections along the Female Reproductive Tract.Infection and immunity · 2018Article
- Neisseria gonorrhoeae employs two protein inhibitors to evade killing by human lysozyme.PLoS pathogens · 2018Article
- Pathogenesis of Neisseria gonorrhoeae in the female reproductive tract: neutrophilic host response, sustained infection, and clinical sequelae.Current opinion in hematology · 2018Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During gonorrhoeal infection, there is a heterogeneous population of Neisseria gonorrhoeae (Gc) varied in their expression of opacity-associated (Opa) proteins. While Opa proteins are important for bacterial attachment and invasion of epithelial cells, Opa+ Gc has a survival defect after exposure to neutrophils. Here, we use constitutively Opa- and OpaD+ Gc in strain background FA1090 to show that Opa+ Gc is more sensitive to killing inside adherent, chemokine-treated primary human neutrophils due to increased bacterial residence in mature, degradative phagolysosomes that contain primary and secondary granule antimicrobial contents. Although Opa+ Gc stimulates a potent oxidative burst, neutrophil killing of Opa+ Gc was instead attributable to non-oxidative components, particularly neutrophil proteases and the bactericidal/permeability-increasing protein. Blocking interaction of Opa+ Gc with carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) or inhibiting Src family kinase signalling, which is downstream of CEACAM activation, enhanced the survival of Opa+ Gc in neutrophils. Src family kinase signalling was required for fusion of Gc phagosomes with primary granules to generate mature phagolysosomes. Conversely, ectopic activation of Src family kinases or coinfection with Opa+ Gc resulted in decreased survival of Opa- Gc in neutrophils. From these results, we conclude that Opa protein expression is an important modulator of Gc survival characteristics in neutrophils by influencing phagosome dynamics and thus bacterial exposure to neutrophils' full antimicrobial arsenal.
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Registered trials
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