ArticleAngewandte Chemie (International ed. in English)2014
In situ vesicle formation by native chemical ligation.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Breaking the membrane heredity paradox through de novo protocell formation.Nature communications · 2026Article
- A synthetic cell with integrated DNA self-replication and lipid biosynthesis.Nature communications · 2026Article
- Construction of Phospholipid-Like Vesicles by Aqueous Aminolysis of Acyl Thioesters With Diamino Acids.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Quantifying the Shape and Stiffness of Single Extracellular Vesicles in Aqueous Solution via Membrane Diffusivity Measurements.Chemical & biomedical imaging · 2025Article
- Photochemical synthesis of natural lipids in artificial and living cells.Nature communications · 2025Article
- Diacylation of Peptides Enables the Construction of Functional Vesicles for Drug-Carrying Liposomes.Angewandte Chemie (International ed. in English) · 2025Article
- Aqueous Synthesis of Membrane Lipids via Amide Formation between Amphiphilic Amines and Thioacids.ChemSystemsChem · 2025Article
- Protocells by spontaneous reaction of cysteine with short-chain thioesters.Nature chemistry · 2025Article
- Biomimetic Materials to Fabricate Artificial Cells.Chemical reviews · 2024Review
- Rapid Formation of Non-canonical Phospholipid Membranes by Chemoselective Amide-Forming Ligations with Hydroxylamines.Angewandte Chemie (International ed. in English) · 2024Article
- Biomimetic construction of phospholipid membranes by direct aminolysis ligations.Interface focus · 2023Article
- Bioconjugation Strategies for Revealing the Roles of Lipids in Living Cells.Accounts of chemical research · 2022Article
- Article
- Genetically controlled membrane synthesis in liposomes.Nature communications · 2020Article
- Lipids: chemical tools for their synthesis, modification, and analysis.Chemical Society reviews · 2020Review
- Traceless native chemical ligation of lipid-modified peptide surfactants by mixed micelle formation.Nature communications · 2020Article
- Budding and Division of Giant Vesicles Linked to Phospholipid Production.Scientific reports · 2019Article
- A minimal biochemical route towards de novo formation of synthetic phospholipid membranes.Nature communications · 2019Article
- The hallmarks of living systems: towards creating artificial cells.Interface focus · 2018Review
- Traceless synthesis of ceramides in living cells reveals saturation-dependent apoptotic effects.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Phospholipid vesicles are of intense fundamental and practical interest, yet methods for their de novo generation from reactive precursors are limited. A non-enzymatic and chemoselective method to spontaneously generate phospholipid membranes from water-soluble starting materials would be a powerful tool for generating vesicles and studying lipid membranes. Here we describe the use of native chemical ligation (NCL) to rapidly prepare phospholipids spontaneously from thioesters. While NCL is one of the most popular tools for synthesizing proteins and nucleic acids, to our knowledge this is the first example of using NCL to generate phospholipids de novo. The lipids are capable of in situ synthesis and self-assembly into vesicles that can grow to several microns in diameter. The selectivity of the NCL reaction makes in situ membrane formation compatible with biological materials such as proteins. This work expands the application of NCL to the formation of phospholipid membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.