ArticleAngewandte Chemie (International ed. in English)2014
Simultaneous in situ quantification of two cellular lipid pools using orthogonal fluorescent sensors.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- PI(3,5)PScience advances · 2026Article
- Probing and imaging phospholipid dynamics in live cells.Life metabolism · 2024Review
- Cell-Permeable Fluorescent Sensors Enable Rapid Live Cell Visualization of Plasma Membrane and Nuclear PIP3 Pools.JACS Au · 2024Article
- Cellular and molecular mechanisms of Hedgehog signalling.Nature reviews. Molecular cell biology · 2023Review
- A Peptide-Based Fluorescent Sensor for Anionic Phospholipids.ACS omega · 2022Article
- An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The chemistry and biology of phosphatidylinositol 4-phosphate at the plasma membrane.Bioorganic & medicinal chemistry · 2021Review
- Photostable and Orthogonal Solvatochromic Fluorophores for SimultaneousACS chemical biology · 2020Article
- A direct fluorometric activity assay for lipid kinases and phosphatases.Journal of lipid research · 2020Article
- Do ABC transporters regulate plasma membrane organization?Cellular & molecular biology letters · 2020Review
- Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched.Cell · 2018Article
- Cholesterol-dependent cytolysins: from water-soluble state to membrane pore.Biophysical reviews · 2018Review
- Quantitative Lipid Imaging Reveals a New Signaling Function of Phosphatidylinositol-3,4-Bisphophate: Isoform- and Site-Specific Activation of Akt.Molecular cell · 2018Article
- Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes.Analytical and bioanalytical chemistry · 2017Article
- Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol.Nature chemical biology · 2017Article
- SH2 Domains Serve as Lipid-Binding Modules for pTyr-Signaling Proteins.Molecular cell · 2016Article
- BRET-monitoring of the dynamic changes of inositol lipid pools in living cells reveals a PKC-dependent PtdIns4P increase upon EGF and M3 receptor activation.Biochimica et biophysica acta · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
Abstract
Lipids regulate a wide range of biological activities. Since their local concentrations are tightly controlled in a spatiotemporally specific manner, the simultaneous quantification of multiple lipids is essential for elucidation of the complex mechanisms of biological regulation. Here, we report a new method for the simultaneous in situ quantification of two lipid pools in mammalian cells using orthogonal fluorescent sensors. The sensors were prepared by incorporating two environmentally sensitive fluorophores with minimal spectral overlap separately into engineered lipid-binding proteins. Dual ratiometric analysis of imaging data allowed accurate, spatiotemporally resolved quantification of two different lipids on the same leaflet of the plasma membrane or a single lipid on two opposite leaflets of the plasma membrane of live mammalian cells. This new imaging technology should serve as a powerful tool for systems-level investigation of lipid-mediated cell signaling and regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.