ArticleScientific reports2014
Reference genes for normalizing transcription in diploid and tetraploid Arabidopsis.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed.
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- Restoring cytonuclear harmony: Distinct strategies in Arabidopsis auto- and allopolyploids.The Plant journal : for cell and molecular biology · 2025Article
- Transcriptomics and trans-organellar complementation reveal limited signaling of 12-cis-oxo-phytodienoic acid during early wound response in Arabidopsis.Nature communications · 2025Article
- Glucosinolates can act as signals to modulate intercellular trafficking via plasmodesmata.The New phytologist · 2025Article
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- PICKLE RELATED 2 is a Neofunctionalized Gene Duplicate Under Positive Selection With Antagonistic Effects to the Ancestral PICKLE Gene on the Seed Transcriptome.Genome biology and evolution · 2023Article
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- Floral Development onPlants (Basel, Switzerland) · 2023Article
- Evaluating and Validating Sunflower Reference Genes for Q-PCR Studies Under High Temperature Condition.Iranian journal of biotechnology · 2023Article
- Overexpression of the elongation factor MtEF1A1 promotes salt stress tolerance in Arabidopsis thaliana and Medicago truncatula.BMC plant biology · 2023Article
- Selection of suitable reference genes for qPCR normalization in different developmental stages ofFrontiers in plant science · 2023Article
- Combined Transcriptomic and Metabolomic Analysis Reveals Insights into Resistance of ArabidopsisPlants (Basel, Switzerland) · 2022Article
- Validation of Reference Genes in a Population of BlueberryPlants (Basel, Switzerland) · 2022Article
- Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana.Plant reproduction · 2022Article
- A peptide-mediated, multilateral molecular dialogue for the coordination of pollen wall formation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Evaluation of Reference Genes in the Polyploid ComplexPlants (Basel, Switzerland) · 2022Article
- Cell division in the shoot apical meristem is a trigger for miR156 decline and vegetative phase transition inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione inInternational journal of molecular sciences · 2021Article
- AtMAD: Arabidopsis thaliana multi-omics association database.Nucleic acids research · 2021Article
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7 authors.
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Abstract
Published transcription data from a set of 19 diploid Arabidopsis thaliana and 5 tetraploid (3 allo- and 2 auto- tetraploid) Arabidopsis accessions were re-analysed to identify reliable reference genes for normalization purposes. Five conventional and 16 novel reference genes previously derived from microarray data covering a wide range of abundance in absolute expression levels in diploid A. thaliana Col-0 were employed. Transcript abundance was well conserved for all 21 potential reference genes in the diploid A. thaliana accessions, with geNorm and NormFinder analysis indicating that AT5G46630, AT1G13320, AT4G26410, AT5G60390 and AT5G08290 were the most stable. However, conservation was less good among the tetraploid accessions, with the transcription of seven of the 21 genes being undetectable in all allotetraploids. The most stable gene was AT5G46630, while AT1G13440 was the unstable one. Hence, the choice of reference gene(s) for A. thaliana is quite wide, but with respect to the analysis of transcriptomic data derived from the tetraploids, it is probably necessary to select more than one reference gene.
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