ArticleScientific reports2014
Toward a more accurate view of human B-cell repertoire by next-generation sequencing, unbiased repertoire capture and single-molecule barcoding.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 88 citations in OpenAlex.
- Accelerating antibody discovery and optimization with high-throughput experimentation and machine learning.Journal of biomedical science · 2025Review
- Beyond glycan barriers: non-cognate ligands and protein mimicry approaches to elicit broadly neutralizing antibodies for HIV-1.Journal of biomedical science · 2024Review
- B-Cell Receptor Repertoire: Recent Advances in Autoimmune Diseases.Clinical reviews in allergy & immunology · 2024Review
- Article
- Applications of High-Throughput DNA Sequencing to Single-Domain Antibody Discovery and Engineering.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor.Cell reports · 2022Article
- Moving beyond Titers.Vaccines · 2022Review
- Resistance of SARS-CoV-2 variants to neutralization by antibodies induced in convalescent patients with COVID-19.Cell reports · 2021Article
- The methods and advances of adaptive immune receptors repertoire sequencing.Theranostics · 2021Article
- GLaMST: grow lineages along minimum spanning tree for b cell receptor sequencing data.BMC genomics · 2020Article
- A VScience advances · 2020Article
- Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans.Nature communications · 2020Article
- Next-generation sequencing and single-cell RT-PCR reveal a distinct variable gene usage of porcine antibody repertoire following PEDV vaccination.Science China. Life sciences · 2020Article
- Systematic Profiling of Full-Length Ig and TCR Repertoire Diversity in Rhesus Macaque through Long Read Transcriptome Sequencing.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Review
- An MPER antibody neutralizes HIV-1 using germline features shared among donors.Nature communications · 2019Article
- Understanding and measuring human B-cell tolerance and its breakdown in autoimmune disease.Immunological reviews · 2019Review
- Deep sequencing of B cell receptor repertoire.BMB reports · 2019Article
- Survival analysis of DNA mutation motifs with penalized proportional hazards.The annals of applied statistics · 2019Article
- Application of long read sequencing to determine expressed antigen diversity in Trypanosoma brucei infections.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
B-cell repertoire analysis using next-generation sequencing has become a valuable tool for interrogating the genetic record of humoral response to infection. However, key obstacles such as low throughput, short read length, high error rate, and undetermined bias of multiplex PCR method have hindered broader application of this technology. In this study, we report several technical advances in antibody repertoire sequencing. We first demonstrated the ability to sequence antibody variable domains using the Ion Torrent PGM platform. As a test case, we analyzed the PGT121 class of antibodies from IAVI donor 17, an HIV-1-infected individual. We then obtained "unbiased" antibody repertoires by sequencing the 5'-RACE PCR products of B-cell transcripts from IAVI donor 17 and two HIV-1-uninfected individuals. We also quantified the bias of previously published gene-specific primers by comparing the repertoires generated by 5'-RACE PCR and multiplex PCR. We further developed a single-molecule barcoding strategy to reduce PCR-based amplification noise. Lastly, we evaluated several new PGM technologies in the context of antibody sequencing. We expect that, based upon long-read and high-fidelity next-generation sequencing technologies, the unbiased analysis will provide a more accurate view of the overall antibody repertoire while the barcoding strategy will facilitate high-resolution analysis of individual antibody families.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.