Evidence map›Paper›PMID 25344811›Full record

ArticleNature chemical biology2014

Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas.

Anne Hong-Hermesdorf, Marcus Miethke, Sean D Gallaher, Janette Kropat, Sheel C Dodani, Jefferson Chan, Dulmini Barupala, Dylan W Domaille, Dyna I Shirasaki, Joseph A Loo and 5 more

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 78 papers.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed
12.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed, 172 citations in OpenAlex.

  1. Article
  2. Article
  3. Simultaneous Imaging of CuAngewandte Chemie (International ed. in English) · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025
    Review
  8. Review
  9. A defined growth medium for trace metal limitation studies inbioRxiv : the preprint server for biology · 2025
    Article
  10. Review
  11. Article
  12. Metals at the Host-Fungal Pathogen Battleground.Annual review of microbiology · 2024
    Review
  13. Review
  14. Review
  15. Article
  16. Lysosome-related organelles contain an expansion compartment that mediates delivery of zinc transporters to promote homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  17. Article
  18. Review
  19. Article
  20. Article

18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 1 country.

Anne Hong-HermesdorfDepartment of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California, USA.
Marcus MiethkeDepartment of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California, USA.
Sean D GallaherDepartment of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California, USA.
Janette KropatDepartment of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California, USA.
Sheel C Dodani1] Department of Chemistry, University of California-Berkeley, Berkeley, California, USA. [2] Howard Hughes Medical Institute, University of California-Berkeley, Berkeley, California, USA.
Jefferson Chan1] Department of Chemistry, University of California-Berkeley, Berkeley, California, USA. [2] Howard Hughes Medical Institute, University of California-Berkeley, Berkeley, California, USA.
Dulmini BarupalaDepartment of Pharmaceutical Sciences, Wayne State University, Detroit, Michigan, USA.
Dylan W Domaille1] Department of Chemistry, University of California-Berkeley, Berkeley, California, USA. [2] Howard Hughes Medical Institute, University of California-Berkeley, Berkeley, California, USA.
Dyna I ShirasakiDepartment of Biological Chemistry, University of California-Los Angeles, Los Angeles, California, USA.
Joseph A Loo1] Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California, USA. [2] Department of Biological Chemistry, University of California-Los Angeles, Los Angeles, California, USA. [3] Institute for Genomics and Proteomics, University of California-Los Angeles, Los Angeles, USA.
Peter K WeberChemical Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.
Jennifer Pett-RidgeChemical Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.
Timothy L StemmlerDepartment of Pharmaceutical Sciences, Wayne State University, Detroit, Michigan, USA.
Christopher J Chang1] Department of Chemistry, University of California-Berkeley, Berkeley, California, USA. [2] Howard Hughes Medical Institute, University of California-Berkeley, Berkeley, California, USA.
Sabeeha S Merchant1] Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, California, USA. [2] Institute for Genomics and Proteomics, University of California-Los Angeles, Los Angeles, USA.
University of California, Los Angeles · USUniversity of California, Berkeley · USLawrence Livermore National Laboratory · USWayne State University · USHoward Hughes Medical Institute · US

Funding

Molecular Imaging Probes To Study Redox BiologyR01GM079465 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Christopher J. Chang · 2006 to 2026
$7.8M
Nutritional Cu Signaling and HomeostasisR01GM042143 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SABEEHA MERCHANT · 1995 to 2026
$4.6M
Nutritional Copper Signaling and HomeostasisR37GM042143 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MERCHANT, SABEEHA · 2011 to 2020
$4.3M
Structural Insights into the Function of FrataxinR01DK068139 · NIDDK · WAYNE STATE UNIVERSITY · PI STEMMLER, TIMOTHY LOUIS · 2005 to 2015
$3.5M
Detroit Cardiovascular Training ProgramT32HL120822 · NHLBI · WAYNE STATE UNIVERSITY · PI LEVY, PHILLIP DAVID · 2014 to 2023
$1.8M
Transcriptional profiling and annotation of the Chlamydomonas genomeR24GM092473 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MERCHANT, SABEEHA · 2011 to 2014
$1.2M
CU-DEPENDENT TRANSCRIPTIONAL REGULATIONR29GM042143 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MERCHANT, SABEEHA · 1989 to 1993
–
Howard Hughes Medical InstituteNHLBI NIH HHS T32 HL120822NHLBI NIH HHS T32HL120822NIDDK NIH HHS DK068139NIDDK NIH HHS R01 DK068139NIGMS NIH HHS GM079465NIGMS NIH HHS GM092473NIGMS NIH HHS GM42143NIGMS NIH HHS R01 GM042143NIGMS NIH HHS R01 GM079465NIGMS NIH HHS R24 GM092473NIGMS NIH HHS R37 GM042143
6 · The paper itself

Abstract

We identified a Cu-accumulating structure with a dynamic role in intracellular Cu homeostasis. During Zn limitation, Chlamydomonas reinhardtii hyperaccumulates Cu, a process dependent on the nutritional Cu sensor CRR1, but it is functionally Cu deficient. Visualization of intracellular Cu revealed major Cu accumulation sites coincident with electron-dense structures that stained positive for low pH and polyphosphate, suggesting that they are lysosome-related organelles. Nano-secondary ion MS showed colocalization of Ca and Cu, and X-ray absorption spectroscopy was consistent with Cu(+) accumulation in an ordered structure. Zn resupply restored Cu homeostasis concomitant with reduced abundance of these structures. Cu isotope labeling demonstrated that sequestered Cu(+) became bioavailable for the synthesis of plastocyanin, and transcriptome profiling indicated that mobilized Cu became visible to CRR1. Cu trafficking to intracellular accumulation sites may be a strategy for preventing protein mismetallation during Zn deficiency and enabling efficient cuproprotein metallation or remetallation upon Zn resupply.

Indexed as

TranscriptomeCations, DivalentChlamydomonas reinhardtiiCopperGene Expression ProfilingHomeostasisHydrogen-Ion ConcentrationIsotope LabelingIsotopesLysosomesMolecular ImagingPlastocyaninPolyphosphatesTranscription FactorsZincCations, DivalentCopperIsotopesPlastocyaninPolyphosphatesTranscription FactorsZinc

Identifiers

PMID25344811
PMCPMC4232477
OpenAlexW2030572315

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.